The Expression And Sense Of Matri Metalloproteinaes-7 And Tissue Inhibitor Of Metalloproteinase-1 In Cervical Carcinoma

Objective: cervical cancinoma is the second most familiar malignancy following mammary carcinoma among women all over the world, and about 200,000 women die of it annually. As is known to all, cervix is consisted of IV collagen, laminin, fibrin combine element and glycosaminoglycans, which are mostly the substrate of matrix metalloproteinase-7, and tissue inhibitor of matrix metalloproteinase-1 is its' inhibitor. Both MMP-7, TIMP-1 and the balance between them play an important role in the arising and expanding of cervical carcinoma. The aim of this research is to investigate the expression of MMP-7 and TIMP-1 in the cervical carcinoma and to evaluate the relationship between the expression of both MMP-7, TIMP-1 and clinicpathologic features of cervical carcinoma in order to seek new prognosis index, and new therapy approach.Methods: The expression and distribution of MMP-7 and TIMP-1 proteins are detected by immunohistochemical technique in 53 cases of cervical carcinoma tissue, 9 cases of CIN and 10 cases of normal cervices tissue coming from operation specimen excised because of myoma of uterus.Classification is done according to the depth of the color and the proportion of the masculine cell.Results: l.The level of MMP-7 and TIMP-1 are significantly higher in cervical carcinoma than in CIN and normal cervices (p<0.01). The protein mainly expresses in the cytoplasm of cervical carcinoma cells. 2. There is a weak relationship between MMP-7 and TIMP-1 (r=0.281, p<0.05). And the expression of MMP-7 is strong in most cases while that of TIMP-1 is weak in most ones.3.The expression of MMP-7 is related to pathological classification, differentiation and lymph node metastasis (p<0.05), and has nothing to do with age, clinical stages (p>0.05). However there is no sign showing the expression of TIMP-1 has something to do with the biological behavior of cervical carcinoma in carcinoma cell (p>0.05).Conclusion: MMP-7 owns its importance in the invasion, metastasis and prognosis of cervical carcinoma, which can act as the new therapy target and be inhibited by TIMP-1.