Inhibition Influence Of Subthalamic Nucleus On EEG And Behavior Scale In Rat Acute Epilepsy Models By High Frequency Stimulation

Objective: To evaluate the effect of different mode of high frequent stimulation in subthalamic nucleus, 5 second phasic stimulation and long lasting continuous stimulation, on EEG and behavior scale of epilepsy rat models induced by penicillin.Methods: 48 male adult Wister rats were randomly divided into three groups: 5 second phasic group, long lasting continuous stimulation group and control group. Stereotactic techniques were used to implant one co-axis electrode in each subthalamic nucleus. After obtaining acute epilepsy rat model by injecting PNC into cortex, we stimulated the bilateral STN in high frequency by relevant stimulation mode. Meanwhile, the EEG and behavior scale grades were recorded in 5 points: pre-stimulation lmin, post-stimulation 1min, 6min, 11min and 16min. With the histological examine of cerebral coronal slices finished, the samples having correct location of electrode tips in both STN were included in our study. Statistical analysis has been conducted on the frequency of spike-sharp wave and discrepancy in behavior grading scale.Results: 1. The rapid onset and stable degree were characteristic of the acute epilepsy rat models induced by penicillin microinjection into cortex.2. The spike-sharp wave frequency in three groups was dissimilar to one another and had statistical significance. 5s short phasic stimulation exerted superior inhibitory effect on seizure to long lasting continuous stimulation.3. The behavior scale in three groups was also dissimilar and had dramatic decrease after high frequency stimulation on STN.Zonclusion: 1. Rat acute epilepsy models induced by penicillin injection into cortex have proved to be stable and replicable.2. STN may be an ideal target in deep brain stimulation therapy for controlling epilepsy.3. High frequency stimulation on both STN does inhibit the generalized tonic and clonic seizure of epilepsy rat models induced by penicillin injection to cortex.4. 5s short phasic stimulation has superior inhibitory effect on GTCS in rats to long lasting continuous stimulation.5. The inhibitory effect on epilepsy by stimulation in STN maybe phasic-dependant, and optimal stimulation mode still needs further research.