Relationship Among The Xba â…  Locus Polymorphisms Of Apolipoprotein B Gene, Serum Lipid Metabolism And Gallstone Disease

Objectives To explore the relationship among the Xba I locus polymorphisms of apolipoprotein B gene , serum lipid metabolism and gallstone disease.Methods Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used to analyze the genotype of the ApoB gene and the serum lipids assayed in 70 patients with gallbladder stones and 43 normal patients as controls .according to the design of case control study.Result The frequencies of X+X - and X-X- of Xba I locus polymorphism were significantly different between the patients and controls and the frequency of X+X - genotype in patients was significantly higher than that in controls(28.6% vs. 11.6%, P<0.05) . There was also a high frequency of X+ allele in the group of patients as compared with the control group (14.3% vs. 5.8%, P < 0.05). Meanwhile , the patients with X+ allele had a significantly higher total serum cholesterol (4.99±0.99mmol/L vs.4.34 + 1.04mmol/L , P<0.05) and higher low density lipoprotein cholesterol(2.88 ± 0.75mmol/L vs.2.34 ± 0.70mmol/L, P<0.01) and the levels of Apolipoprotein B of both serum and bile than those in patients without X+ allele. There was a significant difference between thegallstone group and control in the value of high density lipoprotein cholesterol -. low density lipoprotein cholesterol and Apolipoprotein B of both serum and bile (P<0.05) .Conclusion Xba I polymorphism of Apolipoprotein B gene may be associated with cholesterol gallstone desease. Variation of X+ may affect serum lipid metabolism and formation of gallstone. X+ allele might be the susceptible gene for cholesterol gallstone disease.