The Study On The Mechanism Of Hypertrophic Scars With Treatment Of ～(90)Sr

[Abstract] Objective To study the expression and histological characteristic of apoptosis related gene bcl-2 and Fas, and proliferation cell nuclear antigen (PCNA) in hypertrophic scars treated with ~90Sr for investigating the mechanism of hypertrophic scars with treatment of ~90Sr. Method The morphological change, mean density of fibroblasts ultrastructure and expressive ratio and site of Bcl-2, Fas and PCNA were detected by light and electronic microscopy and immunohistochemistry in 10 cases hypertrophic scars experimental group (scars irradiated by ~90Sr: ~90SrHSG) and control group (scars without treatment of ~90Sr : HSG) and normal skin group( NSG ). Result (l)The expressive site of Fas positive was in cell membrane and cytoplasm of basal keratinocytes and dermal fibroblasts of all HSG and ~90SrHSG .The expressive site of Bcl-2 positive was in cytoplasm of basal keratinocytes and dermal fibroblasts of all HSG and ~90SrHSG and NSG .The positive site of PCNA was in the nucleus of basal Keratinocytes and dermal fibroblasts of all HSG and ~90SrHSG .In dermal fibroblasts: the level of Fas in HSG (21.70±11.42) was significantly lower than ~90SrHSG (38.80±11.27)( P <0.05); the level of Bcl-2 in HSG (35.00±10.14) was significantly higher than ~90Sr HSG (17.50±7.50)( P <0.05); the level of PCNA in HSG (44.50±19.36) was significantly higher than ~90Sr HSG (27.30±8.84)( P <0.05) .In keratinocytes : the level of Fas in HSG (34.40±7.54) was significantly lower than ~90Sr HSG (49.60±16.04)(P<0.05); the level of Bcl-2 in HSG (38.20±12.91) was significantly higher than ~90Sr HSG (25.70±10.10)( P <0.05); the level of PCNA in HSG (53.90±17.15) was significantly higher than ~90Sr HSG (37.50±19.67) (P <0.05). (2)There were significantly differences in morphology between HSG and ~90Sr HSG . The numbers of dermal fibroblasts in HSG (197.60±65.37) was much higher than ~90SrHSG (85.73±20.26) (P <0.05) .The quantities of micrangium in ~90SrHSG was less and its luminawas narrower than HSG. The vascular endothelial cell in 90SrHSG depended to cataplasis. (3) The result of electronic microscope : there were a great ratio fibroblasts in HSG , and many rough endoplasmic reticulum in the fibroblasts, Karyomegaly, and affluent chromatin in the nucleus. In %Sr HSG, the numbers of fibroblasts were less than HSG, parts of its cytoplasm and rough endoplasmic reticulum were insufficiency. Conclusions: The effects of 90Sr on hypertrophic scars were achieved by inhabiting proliferation and biosynthesis of fibroblasts and promoting apoptosis of fibroblasts and keratinocytes.