Differential Expression Of TsRNA In Human Hypertrophic Scar Fibroblast Based On High-throughput Sequencing

Objective:studies have confirmed that tRNA derived small RNA(tsRNA)plays an important regulatory role in many physiological and pathological processes,but their role in hypertrophic scar is still unclear.Methods:the differential expression of tsRNA in human hypertrophic scar fibroblasts and normal skin fibroblasts was revealed by high throughput sequencing and validated by reverse transcription polymerase chain reaction(RT-qPCR).Then,GO analysis,KEGG pathway analysis,target gene prediction,co expression network CNC and competitive endogenous RNA(ceRNA)network were used to explore the possible role of these differentially expressed tsRNA.Results:a total of 67 differentially expressed tsRNAs were detected in hypertrophic scar fibroblasts,of which 27 were up-regulated and 40 were down regulated.These aberrant tsRNAs are related to the biological functions of nervous system development,cell adhesion,protein binding,angiogenesis and actin binding.KEGG pathway analysis showed that the differentially expressed tsrna was involved in Ras,Rap1 and cGMP-PKG and other signaling pathways.The prediction of target genes also indicates that the target genes of tsrna are related to several important signaling pathways of hypertrophic scar formation.The results of RT-qPCR were consistent with those of sequencing.MiR-29b-1-5p,as a target of tsrna-23678,is down regulated in hypertrophic scar fibroblasts and is a negative regulator of scar formation.In addition,tsRNA-23761 can act as a ceRNA and bind to miR-3135b to regulate the expression of its target ace.Results this study revealed the differential expression of tsRNA in human hypertrophic scar fibroblasts,further clarified the molecular mechanism of hypertrophic scar,and provided new ideas and theoretical basis for finding new therapeutic targets.