The Study On Mechanism Of RhIFN-α And RhIL-2 Affect Hypertrophic Scar Fibroblasts

[Objective]:To investigate the relationship between rhIFN-α and rhIL-2 and their effects on biological behavior and pathological changes of fibroblasts, so that the mechanism which cytokines affect hypertrophic scar can be disclose to provide the theory base for clinical prevention and treatment on hypertrophic scar. [Materials and Methods]: Both hypertrophic scar derived fibroblasts cultivated in vitro and Wistar rats injury model derived fibroblasts in vito were observed and analysed through Light microscope(LM), transmission elextron microscope(TEM), MTT test, Mallory's staining and flow cytometry(FCM). Then the effects of rhIFN-α and rhIL-2 on biological behavior and pathological changes of hypertrophic scar fibroblasts have been determined. [Results]: 1. In vitro: compared with the control group, the experimental rhIFN- a group could be observed changes on inhibition of fibroblasts growth and proliferation, decrease of the S-G2-M phase cell percentage and increase of protein expression quantities of Fas while that of Bcl-2 adverse. The experimental rhIL-2 group, enhancement of fibroblast growth and proliferation, increase of the S-G2-M phase cell percentage and higher expression quantities both in Bcl-2 and Fas. 2. In vito: compared with the control group, The rhIFN-α group could be observed changes on inhibition of fibroblasts growth and collagen synthesis and apoptosis promotion . rhIL-2group, enhancement of fibroblast growth, stimulation of collagen synthesis, secretion and accumulation. [Conclusions]: 1. rhIFN-α can suppress growth and proliferation of hypertrophic scar fibroblasts, inhibit collagen synthesis and promote apoptosis. It indicates that,as a negative modulation factor, rhIFN-α has its importance on treatment of hypertrophic scar. 2. rhIL-2 can increase growth and proliferation of hypertrophic scar fibroblasts, stimulate collagen synthesis, secretion and accumulation. It indicates that, as a positive modulation factor, rhIL-2 is beneficial for wound healing at early stage following injury. However, if it acts excessively , it may lead to hypertrophic scar forming.