Expression Of P57～(kip2) And PCNA In Bladder Transitional Cell Carcinoma And Its Significance

Objective: TO study expressions and correlations of p57kip2,PCNA in bladder transitional cell carcinoma(BTCC)and discuss their significance in carcinogenesis and progression of BTCC cancer,as well as their relations with angiogenesis and prognosis. Methods: Fifty specimens of BTCC between 1994 and 1997 at the department of Urology,the First Affiliated Hospital Chongqing Medical University,in the study group.The expressions of p57kip2 and PCNA were tested by immunohistochemical method(EliVisionTMplus). The results were reciprocally analyzed and compared with angiogenesis and prognosis. Results: p57kip2 and PCNA positive stained were a greater or lesser degree detected in all of the 50 cases.Positive rate of p57kip2 in BTCC was 46%,which was significant lower than 78.57% in normal bladder muscosa.(χ2=4.660,P <0.05),p57kip2 expression correlated remarkably with both grade and survival(χ2=8.592,P<0.05;χ2=4.546,P<0.05), but did not correlate clinical stage(χ2=0.573,P>0.05);positive rate of PCNA in BTCC was 60 %(30/50),which was significant higher than 0%(0/14)in normal bladder muscosa(χ2=15.812, p <0.01),PCNA expression correlated remarkably with both grade ,stage and survival(P<0.05)there was a highly significant inverse correlation between the expression of p57kip2 and PCNA(r=-0.443,P <0.01) Conclusion: l. The low express of p57kip2 and overexpression of PCNA are closely related to tumorigenesis of bladder cancer. 2. p57kip2 expression correlated remarkably with both grade and survival,but did not correlate clinical stage,indicating that p57kip2 is related to the differentiation and malignant degree of bladder cancer,not related to tumor infiltration,it has a poor prognosis.PCNA expression correlated remarkably with both grade ,stage and survival, indicating PCNA are related to the differentiation and progression of bladder cancer and it might be useful prognosistic markers in bladder carcinoma. 3.There was a negative correlation between the expression rate of p57kip2 and PCNA in bladder cancer, indicating that they have synergism in the happening and development of bladder cancer.At the same time they may have some feedback loop in progression of bladder cancer.