The Study Of Retinal Ganglion Cells Of Microtubule Associated Protein-1B Following Optic Nerve Crush In Rat

Objective: To study the neuroprotective effects of venom nerve growth factor on retinal ganglion cells after optic nerve crush in rat by observing microtubule associated protein -1BMethods: Forty wistar rats were divided into experimental and experimental control groups at random. The rats optic nerve were crushed by using mini-vascular nip. After crushed the optic nerve of the right eyes, 100U / 0.025ml of vNGF were injected into the vitreous of experimental group injury eyes. 0.025ml balance salt solution were injected into the vitreous of experimental control group injury eyes. The left eyes were not treated with anything as control group. In 3days, 7days, 14days, 30days, 60days after injury, the histological changes of the retina were observed. The numbers of the RGCs were counted by using microscope. The changes of the retina were observed by using electronic microscope, then immunohistochemical staining and analysis the microtubule associated protein -1B.Results: The numbers of RGCs in the three groups were compared by using microscope. In 3-30 days after injury, the numbers of RGCs in treatment group were higher than that in the experimental control group. The differences between the treatment and experimental control groups were significant (P<0.01). After injury, the numbers of RGCs in the experimental control and experimental groups were lower than control group. Their differences were significant (P<0.01). In 60 days after injury, the differences between the numbers of RGCs in the treatment group and experimental control group were not significant(P>0.05). The changes of the retinal were observed by using electron microscope. In 14 days after injury, the numbers of microtubules in the treatment group were higher than those in the experimental control group obviously. The results of...