| Objective : To investigate the neuroprotective effects of venom nerve growth factor on retinal ganglion cells after optic nerve transected in rat by observing microtubule associated cytoskeletal proteins expression changes.Methods : 48 SD rats were randomly divided into vNGF and the control group with 24 rats in each group. After the optic nerve of the right eyes was transected, 100BU/0.025ml of vNGF( NGF group) and 0.02 ml of BSS(control group)were injected into the vitreous of right eyes respectively. The left eyes were not treated with anything as normal control group. Animals were sacrificed at 4 days,7 days and 14 days after the injury.the histological changes of the retina were observed by light microscopy. A computer—image—analysis system was used to count the numbers of the RGCs and to observe the changes of the retina. The expression of cytoskeletal proteins was studied by Immunohistochemistry.Results : The numbers of RGCs in the three groups were compared by using microscope. In 4-14 days after injury, the numbers of RGCs in treatment group were higher than that in the experimental control group. The differences between the treatment and experimental control groups were significant (P<0.01). After injury, the numbers of RGCs in the experimental control and experimental groups were lower than the normal control group. Their differences were significant (P<0.01). The results of immunohistochemical staining: expression of MAP-1B,neurofilaments,tau,GAP-43 in the treatment groups were gradually increasing during two week, but expression of MBP decreasing obviously at the same time.Conclusion: After optic nerve transected, vNGF are effective in enhancing and prolonging the expression of cytoskeletal proteins, increasing the survival rate of RGCs, and has the protection effect on retinal ganglion cells.
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