The Effects Of Venom Nerve Growth Factor On GAP-43 Of Retinal Ganglion Cells Following Optic Nerve Transected In Rat

Objective :To study the neuroprotective effects of venom nerve growth factor on retinal ganglion cells after optic nerve transected in rat by growth associated protein-43.Methods :Twenty four SD rats were divided into experimental control groups at random. The rats optic nerve of the right eyes were transected. After transected , 100BU/0.025ml of vNGF were injected into the vitreous of experimental group eyes. 0.025ml balance salt solution were injected into the vitreous of experimental control group eyes. The left eyes were not treated with anything as normal control group. 7days, 14days after transected, the histological changes of the retina were observed. The numbers of the RGCs were counted and the changes of the retina were observed by using image diagnosis system.The expression of GAP-43 was studied by immunohistochemistry.Results : The numbers of RGCs in the three groups were compared.At 14 days of optic nerve transected, the numbers of RGCs in the experimental group were higher than that in the experimental control group. The differences between the experimental and experimental control groups were significant (P<0.01).the numbers of RGCs in the experimental control and experimental groups were lower than normal control(P<0.01).the results of immunohistochemical staining : expression of GAP-43 in the experimental...