| Objective: By establishing the animal model of liver cirrhosis, then we studied the effects on the levels of Mn, Zn, Cu, Fe, Ca and Mg in the blood, liver and different brain area (Cerebral cortex, Cerebellum and The tissue under cerebral cortex) of rats with liver cirrhosis, as to understand the regulation of concentration change about Mn and the other 5 kinds of metal elements in blood, liver and brain and open out the relation between cirrhosis, manganese and the encephalopathic, then in order to enrich the pathogenesis theory of chronic liver encephalopathic (CHE ) and offer scientific basis for the fact that prevent and cure CHE clinically. Methods: One hundred and twenty rats were randomly divided into three administered groups and three normal control groups. Each group mentioned above was redivided into three subgroups. They were the nine-week group,the twelve-week group and the fifteen-week group, respectively.There was thirty rats in every administered group and ten rats in every normal control group at the same time. It was compound factor (CCl4 + ethanol) that prepared rat's liver cirrhosis model. The rats of control group were injected with physiological saline by hypodermic injection, the frequence and dosage were the same as the administered group. In the sixty-third day, the eighty-fourth day and the one hundred-fifth day of experimentation, rats were paid to some treatments separately, at the same time, collecting the blood, liver tissue and different brain area tissue (Cerebral cortex, Cerebellum and The tissue under cerebral cortex) of all rats, and then adopted the inductively coupled plasma - atomic emission spectrophotometry (ICP-AES) to determine the rat's concentrations of manganese (Mn) and the other 5 kinds of metal elements (Zn , Cu , Fe , Ca and Mg ) in the sample mentioned above. In addition,liver was cut into slices and dye with HE. Results: (1) HE dye showed that three administered groups of rats presented liver cirrhosis pathology that degree aggravate gradually change with contamination. (2) Effects of liver cirrhosis on the level of Mn in rats'blood,liver and brain: The concentrations of Mn in whole-blood of rats in administered group was obviously higher than control group in the nine-week group and the twelve-week group, respectively (P<0.01), There was not a significant difference with control group in the fifteen-week group (P> 0. 05); In the liver tissue, with the aggravating of liver cirrhosis degree, its Mn concentration presented gradient decline; In the cerebral cortex tissue, the concentrations of Mn of rats in administered group was obviously higher than control group in the twelve-week group. (P<0.05); In the tissue under cerebral cortex, the concentration of Mn of rats in administered group was obviously lower than control group in the nine-week group (P<0.01). Concentration of Mn in the others tissue did not have a significant difference. Aggravating with liver cirrhosis degree, the order of Mn concentration in brain tissue from the order—Cerebral cortex >Cerebellum >The tissue under cerebral cortex turned into the order—The tissue under cerebral cortex > Cerebral cortex > Cerebellum.The Mn concentration in the tissue under cerebral cortex inclined to occupy the first place in the three brain areas. (3) Effects of liver cirrhosis on the level of Zn , Cu , Fe , Ca and Mg in rats' blood,liver and brain: Compared to the control group, the concentration of the 5 kinds of metal elements in the blood,liver and brain of rats in administered group happened various change and liver cirrhosis had effects on the distribution in the blood,liver and brain, but aggravated with liver cirrhosis degree, such changes were not obvious.(4) The concentration of these metal elements among blood, liver and brain had different relation. In the same area, there was a various relation between Mn and the other 5 kinds of metal elements. Conclusion: At the time of the liver cirrhosis, the concentration and distribution of these six metal elements in different tissue had been influenced. Th... |
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