Study Of Correlation Among Expression Of DcR3,c-myc,bcl-2 And PTEN Gene In Leukemia

Objective :To study the relationship of the expression of DcR3 genebetween leukemia and control group, the correlation among DcR3 ,c-myc,bcl-2 ,PTEN,to search for mechanism of leukemia .Methods : 30 patients with leukemia diagnosised by MIC , and 14persons as controls,10 healthy volunteers.Cells were isolated by the densitygradient centrifugation method. Total cellular were washed 3 times by coldsaline then use degeneration Burst Fluid Trizol Chloroform isopropanolextract the total RNA ,reverse transcripted to synthesis cDNA . Specificprimers were used to amplify the fragments of our genes and β-actingenes.After electrophoresis on 1.5% agarose gel with ethidium bromide ,theproducts gel images were obtainted and densities of the products werequantified using Tannon GIS gel electrophoresis image analysis system. .therelative expression level of DcR3 gene was calculated as the products of theDcR3 gene divided by that of the β-actin gene .The data were handled usingT-test , analysis of variance by Spearman.Results :The expression of DcR3 ,c-myc,bcl-2 gene that wasdetected from the patients with leukemia was elevated more significantlythan that from the control group. DcR3 was significant correlated withc-myc and bcl-2.Discussion : Recently the incidence rate of leukemia is increasesing ,so it is very important to study its mechism.Because pathways ofFas/FasL,LIGHT/TR2,LIGHT /HVEM and LIGHT/ LTβR are key point actions in the course of immune system apoptosis regulation, itmaybe stand up extremely important action in occurrence and developing of leukemia. Fas, named Apo-1 or CD95,is a type I membrane protein and widely distributes as mFas(membrane from Fas)on the surface ofactivated T cells,B cells and monocytes. FasL,called CD95L,is a typeⅡmembrane protein and also called death factor. It belongs to the TNF(tumour necrosis factor)family and distributes on the surfaces of activatedT cells,B cells,monocytes,immune-privilege-site cells and tumor cells. The FasL can induce the cells expressing Fas to apoptosis by ligation with Fas on surfaces. Very recently, a soluble decoy receptor, termed decoy receptor3(DcR3) ,which binds FasL ,LIGHT to block the Fas/Fas,LIGHT/TR2,LIGHT /HVEM and LIGHT/ LTβR pathway and inhibits FasL and LIGHT-induced apoptosis, has been identified. Further studies show that there are functional similarities between DcR3-Fc and Fas-Fc.They both block the Fas/fasL so to inhibit the AICD of mature T lymphocytes and reduce the killing of Jurkat T leukaemia cells by nature killercells.Therefore, it is possible that over-expression of DcR3 connects with the onset and progress of autoimmune diseases.When DcR3 binds LIGHT competition with TR2 or LTβR to block the LIGHT/TR2,LIGHT/HVEM and LIGHT/ LTβR pathway and inhibits LIGHT induced apoptosis, cell proliferation, cell differentiation, yield of cytokine, synergy stimulation of Lymphocyte and isotype switching; Otsuki.T has proved that DcR3 gene was over-expressed in PBMC from patients with SLE or silicisis, whitch suggested that change of DcR3 expression may play an important role in the pathogenesis of these diseases. In this research, We detected the relative expression of DcR3 ,c-myc ,bcl-2,PTEN in BMNC from the patients with Leukemia and the controls bysemi-quantitave RT-PCR,and investigated the relation between the expressionevel of DcR3 gene and Leukenia. Our results showed that DcR3,c-myc,bcl...