Application Of High Performance Liquid Chromatography/Electrospray-Mas Spectrometry In The Determination Of Several Drugs And Corresponding Metabolites In Human Plasma

Pharmacokinetics, an important branch of pharmacology, takes plasma concentration-time curve (AUC) as its focus of study. The drug's concentration in plasma can reflect its status in target organs and many research works are available for reference. Usually plasma is used as the sample in pharmacokinetics study. Because of the powerful separating ability of high performance liquid chromatography (HPLC), the high sensibility and specificity of mass spectrometry (MS), and the detailed information of target compounds' structures obtained by collision induced dissociation (CID) or MS/MS, HPLC/MS technique is playing a more and more important role in pharmacokinetics study. Nowadays, many drugs, which have good therapeutic efficacy with small dose, are of high polarity and difficult to volatilize. In addition, with the development of biotechnology, bioactive compounds of strong polarity, such as polypeptide and high-molecular-weight proteins, are widely used to treat diseases. As a result, the HPLC/ESI-MS technique, which is effective in analyzing the above-mentioned compounds, has been brought under the spotlight.The studies included in the thesis are as follows:1. To develop an analytical method for simultaneous determination of benazepril, a prodrug-type angiotensin-converting enzyme (ACE)...