Oligonucleotide Microarray For Typing Of The Human Papillomavirus

BackgroundThe human papillomavirus belongs to the Papovaviridae family and the HPV genome consists of a single molecule of double-stranded, circular DNA containing approximately 8,000 bp .Now, more than 200 types of HPV have been recognized .The infection of HPV is the main cause of the cervix and HPV is a necessary factor for the precancerosis and the development of invasion cancer of the cervix. Since the pathogenic mechanism and carcinogenic risk are different for different subtypes of HPV, it is necessary for us to type them .Now, the clinical method of detecting HPV is real-time PCR. But this method can detect only one subtype in one reaction and can't satisfy the need of typing multi-subtype at the same time .In this study, we investigate the possible of typing HPV with oligonucleotide microarray at one time.ObjectiveTo establish mammalian cell clonings involved HPV subtype L1 gene fragment. Based on it , the method should be established initially to type HPV by oligonucleotide chip.Methods1.Establish mammalian cell clonings involved HPV subtype L1 gene fragment as positive control.2.Collect sequence data of HPV subtype from the NCBI database, then design the probes respectively combine with the sequences of our clone.3.Use real-time PCR and oligonucleotide chip to type HPV .Results1.We have cloned 30 HPV subtype L1 gene fragment correlated with cervix cancer and 6 mammalian cell clonings involved HPV subtype L1 gene fragment .2.We have constructed a kind of oligonucleotide array which could type the HPV and established the corresponding technical method.