The Toxicity Comparison And The Mechanism Initial Research On Aconite Root Treating Animal Model Of Yang Asthenia And Yin Asthenia

Purpose:To establish the hypothesis about poison theory by differentiating of symptoms and signs, we take aconite root as the example, study the poisonous differece of all kinds of symptoms which is produced after using aconite root. Copy the yang asthenia and the yin asthenia model of SD mice, observe the poisonous differece poduced by aconite root to the SD mice, and approach the mechanism, thereby check the hypothsis about poison theory by differentiating of symptoms and signs.Methods:To copy the yang asthenia modelof SD mice by intramuscular injection CORT, the yin asthenia model by intragastric administration thyroxine. At same time, give mice aconite root by intragastric administration at low,high dose, observe general condition of every group mice (body weight, temperature), cardiac electrophysiology(heart rate, cardiac rhythm), determine Na~+-K~+-ATP enzyme activity of the cardiac muscle and brain tissue, hepatic-renal function, organ coefficient of important organs, and histomorphology changes, and compare the results.Results:1. Aconite root influence the temperature and body weight of mice, and the influence build up with the dose increase. When give mice aconite root as low-dose group, the yang asthenia model mice hardly produce significant change, while theyin asthenia model mice's temperature increase significantly. As to body weight, the tendency is similar; when it comes to high-dose aconite root group, the temperature and body weight of every group mice all decrease significantly.2. Aconite root influence the heart rate and cardiac rhythm of mice, and theinfluence build up with the dose increase. When give the yin asthenia model group mice aconite root, the mice's heart rate step up, the incidence rate of arrhythmogenesis is high;and the yang asthenia model group take on the same rule, but the results is no more severe than the resuts of yang asthenia group.When give aconite root to mice, the heart coefficient of every group mice all increase, but the increase amplitude of heart coefficient in the yang asthenia group mice is lower than the one of yin asthenia group. From histomorphology, observe the slight injure got from aconite root in every group. At same time, we can get the similar results from liver,kidney,spleen, adrenal, didymus coefficient.3. Aconite root influence Na -K -ATP enzyme activity of the cardiac muscle and brain tissue conspicuously, and this influence built up with dose increase. When give mice aconite root at low dose, the Na+-K+-ATP enzyme activity of yang asthenia model mice has the degression tendency, while the one of yin asthenia group decrease significantly; When give mice aconite root at high dose, the Na+-K+~ATP enzyme activity of cardiac muscle and brain tissue in every group all degrade conspicuously.4. Aconite root influence hepatic-renal function of SD mice conspicuously, and this influence built up with dose increase. When give aconite root to the yin asthenia model mice, AST, ALT, CR, BUN all step up conspicuously, the increase of above index in yang asthenia model group is lower the in the yin asthenia model group.Conclusion:1. the toxic reaction of aconite root can display from body temperature, body weight, the physiological function of heart, liver, kidney and brain, and histomorphology; about it's mechanism, one maybe is the influence Na+-K+-ATP enzyme activity in the cellular membrane.2. As to the above index, there are certain difference between the two kinds group mice which are yin asthenia and yang asthenia model groups. And this hint that in the different syndrome condition, body react to the toxicity produced by aconite root differently, so this experiment can check the hypothesis about poison theory by differentiating of symptoms and signs at certain extent.