Expression Of Survivin And CyclinD1 And Their Relationship With Cell Proliferation And Apoptosis In Gastric Carcinogensis

Objectives: We have aimed to study the expression of Survivin and CyclinDl in gastric cancer and to evaluate the significant correlation between their expression and cell apoptosis and proliferation, to investigate their possible roles in the gastric carcinogenic mechanism, to provide evidence for prevention and therapy of gastric carcinoma using Survivin as a therapeutic target.Methods:Gastric tissue samples were obtained from 12 cases with chronic superficial gastritis(CSG), 32 cases with intestinal metaplasia(IM), 32 cases with dysplasia (Dys) and 50 cases with gastric carcinoma(GC). Expressions of Survivin? CyclinD1 and Ki-67 were dectected by immunohistochemistry (S-P method) , cell apoptosis by TUNEL method and expression of Survivin mRNA by In Situ Hybridization (ISH) technique. The data were analyzed by software SPSS 10.0, chi-square test was used to compare the difference between groups. A difference was considered significance if the p value was below 0.05.Results: Expression of Survivin and CyclinDl showed a gradually increasing tendency from CSG, IM, Dys to GC. Positive expression rates of Survivin and CyclinDl were 16.7% and 8.3% in CSG, 21.9% and 15.6%in IM, 37.5% and 34.4% in Dys, and 54.0% and 48.0% in GC respectively. There was a significant difference between GC and CSG, GC and IM in expression of Survivin (p<0.05); but no significant difference in other groups(p>0.05). There was a same significance of expression of CyclinDl in CSG, IM, Dys and GC. In gastric carcinogensis, apoptosis index was (4.1±0.5)%, (6.1±0.7)%, (3.4±0.4)%, (2.3±0.3)% in CSG, IM, Dys, and GC respectively, and there were significant differences between GC and other groups(p<0.05). However, proliferation index showed an increasing tendency in gastric carcinogensis, (6.2±1.3)%, (19.8 ± 2.6)%, (32.5±2.8)%, (58.4 ± 6.9)% in CSG, IM, Dys, and GC respectively. There was a negative correlation between survivin and AI (r=-0.864, p<0.05), but no correlation between survivin and PI.