PD98059, A Selective Mek Inhibitor, Effect On The Proliferation And Apoptosis Of HepG2 Cell

objective: To investigate the effects of MEK inhibitors on the proliferation and apoptosis of HepG2 cell and its relevant mechanism.Methods: human hepatoma cell line HepG2 trained outside the body, the effectsof MEK inhibitors (PD98059 ) on the growth and survival of human hepatoma cell line HepG2 were determined with microculture tetrazolium assay (MTT), and colony-forming assay and apoptosis and cell cycle analysis was determined using HE staining and flow cytometry.Measured the protein expression of Caspase-3, p53 with immunohistochemistry. Western blot analysis were employed to observe the effec to PD98059 on the expression of pERK protein.Results: PD98059 had marked suppressive effect on the growth of HepG2 cellline.PD98059 can enhance suppressive effect of 5-FU to HepG2 cell.The cells were treated with different concentrations of PD98059(25,50,)umol/L and PD98059 unite 5-FU for up to 72 hr, Cell proliferation was measured using MTT. The inhibitory rates of the cells were 49.03±l .560% 71.48±1.522%> 83.48±1.011% respectively in 72 hours. Colony-formating rate of HepG2 cells were32.0±2.554 %,13.4±1.778%, 7.4±0.751% respectively, which were much lower than that of control group49.4±6.023 (%). At the same time, PD98059 could induce the apoptosis of HepG2 with the cell cycle arrested on G₁/S phase. In immunohistochemistry and western blot analysis, PD98059 could inhibit the protein expression of p-ERK and P53 in human hepatoma HepG2 cells, but the protein expression of Caspase-3 in HepG2 cells were upregulated.