The Matrix Metalloproteinase-2 And The Tissue Inhibitor Of Matrix Metalloproteinase Type-1 In A Rat Model Of Chronic Cyclosporine Nephropthy

[Objective] To explore the role of the tissue inhibitor of matrix metalloproteinase type-1 and the matrix metalloproteinase-2 in a rat model of chronic cyclosporine nephropathy.[Methods] Chose health Wister rats and divided them into four groups at random.one group as the control ,the other three groups treated with different dose of cyclosporine A (10mg/kg, 15mg/kg, 20mg/kg). They were fed on a sodium-deficient diet during the whole period of four weeks. After four weeks of treatment, the rats were sacrificed, the kidney was snap-frozen in liquid nitrogen and stored at -80 °C until RNA extraction was performed. The expression of mRNA of the tissue inhibitor of matrix metalloproteinase type-1 and the matrix metalloproteinase-2 were detected by the RT-PCR.[Results] (1)The expression of mRNA of the tissue inhibitor of matrix metalloproteinase type-1 increased(P < 0.05), and following the increase of the dose of cyclosporine A the expression of mRNA added. (r=0.85)(2)The expression of mRNA of the matrix metalloproteinase-2 increased. (P < 0.05) (3) After the experimentation those rats treated with CsA were lighter than others, and those treated with the highest dose of CsA were lightest. (P < 0.05) (4)The Scr of treated groups increased obviously to control group, but the difference of the Scr among those treated groups was not obvious.(5)The CsA in the blood increased followed withthe increase of the dose of CsA. (P < 0.05)[Conclusion] The increases of the tissue inhibitor of matrix metalloproteinase type-1 mRNA showed that the tissue inhibitor of matrix metalloproteinase type-1 contributed to the development of CsA-infused focal interstitial fibrosis in the rat.And the increase of the matrix metalloproteinase-2 mRNA was the reaction of the change of the extracellular matrix.