| Object The purpose of the present study was to determine the effects of epidermic growth factor (EGF) on survival of the ischemic skin flap in rats. In addition, discussed some probable way that may contribute to this effect.Method A dorsal flap model (2×8cm) was used in this study. In Parti, fourteen male Sprague-Dawley rats were individually assigned to two groups (n1=8, n2=6). Exogenous EGF (15ug/kg) was injected subdermally into the flaps in 8 rats before the flaps were replaced. Flaps that received a saline injection were used as the controls. The skin paddle survival was measured on postoperative day five and ten. Then the phenomenons of neovascular formation was observed, In Part 2, eight male Sprague-Dawley rats were randomly divided into two groups (n=4 in each group). Biopsies were taken from the flap at 2, 4 and 6cm distances from the distal edge at 24 h after the flaps were sutured. Malonyldialdehyde (MDA) level was measured.Result The flap mean viable surface area for the control group was 50.44 ±3.03%(55th day) and 51.43±5.27%(10thday). The group with injection of EGF had a mean viability of 65.57 ±7.01%(5th day) and 64.48 ± 5.76% (10th day).Statistically significant differences were obtained in treatment groups in comparison to the control group (p<0.01). The vascular density in the experimental group was higher than in the control group. There was nosignificant difference in MDA level in rats treated with EGF compared to the control group.Conclusion This is the first time to elucidate that administration of exogenous EGF could significantly enhance survival of ischemic flaps. It may possibly through induction of vessel growth to get this effect. |
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