Protection Effect Of Sodium Ferulate Against Oxidative Stress Induced By Chronic Cerebral Ischemia In Rats

Induction: Chronic cerebral ischemia is a common pathological status, it accompanied with many cerebrovascular diseases, such as cerebral arteriosclerosis, Vascular dementia, Alzheimer's , Arteriovenous malformation. Cognitive impairment is the main clinical situation in earlier period of chronic cerebral ischemia, and then the permanent neural impairment. The pathological mechanism of which is different from acute cerebral ischemia. Oxidative stress is an important vital process, which can help body to accommodate stress, and transduct signal in vivo, Meanwhile it can also induce oxidative damage. Oxidative stress may play an important role in age-related neurodegenerative disorders. It increases in aging brain, participates in many physiological and biochemical procedures. Brain tissue is especially vulnerable to oxidative stress owing to its high oxygen using. So understanding the relationship between chronic cerebral ischemia and oxidative stress can help us to investigate the pathological mechanism of chronic cerebral ischemia and provide method for the therapy and prevetion of the disease.Objective:1. Observed malondialdehyde(MDA) levels, superoxide dismutase(SOD) activities, and protein of poly(ADP-ribose)Polymerase(PARP) from hippocampus of chronic cerebral ischemia in rats.2. Visualize whether sodium ferulate can protect brain of rat against oxidative stress induced by chronic cerebral ischemia.Method:1. The rats were divided into three groups: sodium ferulate treatment group, saline administration group and sham operation group.2. Permanent occlusion of bilateral common carotid occlusion (2VO) was used to induce reduction of cerebral blood flow to the brain of rat. Sodium ferulate treatment group were treated with sodium ferulate at a dose of 50mg/kg given by intraperitoneal injection once daily for ten days after 2VO surgery completed. Saline administration group were given saline solution 0.9% during the same time. Sham operation group were given no treatment.3. Using SOD and MDA Test Kit (nanjing jiancheng bioengineering institut) to testlevels of MDA and activities of SOD in hippocampus.4. Protein level of PARP were measured using Western blotting. Result:1. PARP protein expression in hippocampus of the sodium ferulate administrated group were up-regulated, compared to that of the sham operation group, but decreased compared to the saline treatment group.2. MDA levels of hippocampus in saline administrated group were higher than that of the sham operation and sodium ferulate administrated group, and MDA levels increased in sodium ferulate administrated group campared to sham operation group.3. SOD activities in hippocampus of sodium ferulate administrated group were up-regulated than that of saline administrated group, but reduced than that of sham operation group.Conclusion: Chronic cerebral ischemia produces oxidative stress and disturbs intracellular redox regulation. Sodium ferulate can clear oxygen-derived free radicals, protect brain against oxidative DNA damage induced by ischemia in rats.