Vascular Endothelial Growth Factor On The Function Of Endothelial Progenitor Cells From Peripheral Blood

AIMTo investigate the effect of VEGF on the biology function of peripheral endothelial progenitor cells (EPC) and find the suitable concentration to promote the growth of EPCs. METHODSTotal mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation , and then the cells were plated on fibronectin-coated culture dishes. After 4 days of culture , attached cells were incubated with VEGF in a series of concentrations (0 , 10 , 20 , 50ng/ml) for 72h. Then attached cells were characterized with immunohistochemistry. EPC proliferation , migration activity wereassayed with MTT assay , modified Boyden chamber assay , respectively. EPC adhesion assay was performed by replating MNCs on fibronectin-coated dishes, and then counting adherent cells. RESULTSSuccessfully isolate the EPCs from MNCs and can be differentiated to ECs. Incubation of isolated human MNCs with VEGF increased the proliferative, migratory and adhesive capacities of EPC, and this effect was most prominant when VEGF concentration was 20ng/ml after 72 hours On the same concentration of VEGF, the functions of EPCs from patients with masculine coronary arteriography are lower than that of EPC from patients with negative coronary arteriography. CONCLUSIONEPCs' functional activities are decreased in patients with masculine coronary arteriography. The results suggested that the low concentration of VEGF can improve EPCs' augmentation and its functional activity.