Interleukin-1 Polymorphic Genes Decrease The Risk Of Reflux Esophagitis In Chinese

OBJECTIVE: To evaluate the association between interleukin-1(IL-1) loci polymorphisms and decreased the risk of reflux esophagitis in samples from northern Chinese population.METHODS: Gastric mucosa from 134 patients with reflux esophagitis and 182 controls with chronic gastritis were collected. Genomic DNA was extracted from gastric mucosa and polymorphisms at -31(C to T), -511(C to T) and at intron 2( 86-bp VNTR) of IL-IRN were genotyped by PCR-RFLP and PCR. Hp was identified by rapid urease, ¹⁴C-breath test and giemsa-staining.RESULT: Of the 134 GERD patients, 44(32.8%) were Hp positive, and among the 182 controls, 114(62.6%) were Hp positive, there was a significant difference in the two groups. Three kinds of polymorphism of IL-IRN were found as L/L,L/2,2/2, and the frequencies in patients were 78%,14.3%,7.7% , respectively, however, the frequencies in controls were 55.4%, 25.5%,19.1%. IL-1RN*2 alleles were negatively associated with GERD. The polymorphisms of IL-1B-31 alleles were T/T, C/T, C/C .The frequencies in patients were 53.7%, 29.9%, 16.4% and in controls were 17.6%, 26.4%, 56%o .IL-1B-31 C/C genotype was inversely associated with GERD. IL-1B-511 alleles were T/T, C/T, C/C . The frequencies in patients were 25.4%, 55.2%, 19.4%, and in controls were 23.1%, 60.4%,16.5%. No significant differences were observed between cases and controls in each genotype.CONCLUSION: There is a lower Hp infection in the GERD. In Chinese population , IL-1B-31 and IL-1RN*2 allele polymorphisms were inversely associated with GERD. This study provides evidence supporting the independent protective role of IL-1B-31C/C and IL-1RN*2 allele polymorphisms against GERD.