Matrix Metalloproteinases 9 In Differential Diagnosis Between Renal Cell Carcinoma And Renal Transitional Cell Carcinoma

ObjectRenal tumor is a common disease in urological tumor, whereas most of them are malignant. In primary renal carcinoma, renal cell cancer (RCC) occupies 80% , renal transitional cell carcinoma (TCC) 8%. Renal cell cancer patients' clinical symptoms are very diversity, especially after the invasion into pelvis, it is very difficult to perform different diagnosis with TCC. For the different operation way between RCC and TCC, this differential diagnosis is very important to clinical treatment.Nowadays there are many articles about tumor markers in body fluid, the research about urology cancer is mainly in urine markers. As renal pelvic carcinoma is a transitional cancer, the level of tumor marker in the urine is quitely high. Matrix metalloproteinase 9 ( MMP - 9) is known as Ⅳ gelatinase mainly to degradate basement membrane type Ⅳ collagen. Basement membrane is first barrier against the invasion and diversion of epithelial tumor. A recent study demonstrates there is about 71 percent MMP - 9 enzyme precursor analysis in the perfusion fluid of bladder in BTCC patients. Meanwhile, there is abnormal expression of MMP -9 in RCC tissues. However, the MMP -9 level of RCC and TCC in urine and blood samples are still not researched.Materials and Methods1. Sample collections:this study include 41 patients, all take both urine and blood samples, inwhich there 17 cases of renal cell carcinoma, 14 cases of renal transitional carcinoma and 10 cases of normal persons.2. Methods;After dealing samples with standard way, the samples are kept within deep freezer. And do the experiment all together. We use ELISA to test MMP - 9 in urine and blood samples. SPSS 11.0 for windows is used to analysis the data.Results1. MMP-9 in blood samples;In 41 cases of blood samples, the data in different groups are not different in statistics.2. MMP - 9 in urine samples;MMP - 9 value in the TCC group and RCC group is obviously higher than control group. (P <0. 01) It is different between TCC group and RCC group. (P < 0. 01)3. Blood MMP - 9/urine MMP - 9 value;There is not different among TCC group RCC group and the control group (P >0. 05).4. Amalgamate TCC group and RCC group to the cancer group, the urine MMP - 9 value and blood MMP - 9/urine MMP - 9 is obviously different with control group, but no difference is shown in the blood MMP —9 value.Conclusions1. There are no different between renal TCC and RCC and normal persons in MMP -9 in blood samples.2. MMP - 9 in urine samples of renal transitional cancer is obviously higher than in the urine of RCC patients and normal persons. MMP -9 is a good urine tumor marker for TCC, and can be used to make diagnosis of TCC and its differential diagnosis with RCC.