Relationship Between The Expression Of VEGF And PTEN In Grades Of Glioma

ObjectiveIn the forward study, we have known that the growth of noumenal tumors depended on the growth of new vessels , and the most important factor of the growth of new vessels was vascular endothelial growth factor (VEGF) which directly stimulated cellular fission , proliferation and also was a highly characteristic factor to induce the generation of new vessels. Phosphatase and tensin homolog deleted on chromosometen(PTEN) , as an antioncogene , might affect on the occurrence , proliferation , adhere , invasion , apoptosis of tumor. In glioma , the effect of PTEN might be regulating cell cycle , cell adhesion , invasion , va-siformation , and so on . In this study , we used SP which was a method of im-munohistochemistry to detect the expression of PTEN and VEGF in the samples of glioma of 46 cases. In order to offer information in analysising the relationship between two factors and pathological grades of glioma.Material and MethodThe samples of glioma were taken from department of pathology in 1 st hospital C. M. U. including 29 males ,17 females, mean age was 22 ages to 70 a-ges, pathological grading was classified according to the standard pathological grades for glioma. GradeⅠ 13 cases, Grade Ⅱ 15 cases, Grade Ⅲ 10 cases, and Grade IV 8 cases. The method was SP which was a method of immunohisto-chemistry . all pathological slices were cutted into serial sections of 4 μm, and we took one group to dye as HE to verify the pathological grades of glioma, and then we used SP to detect the expression of PTEN and VEGF. The following upwas what we have done. Paraffin section was deparaffinaged , adding 3% H2O2to them in room temperature for 10 minutes, trypsinization juice for AG reparation of VEGF for 5 minutes, high pressure for PTEN for 2 minutes 30 seconds, washing by PBS for 3 times, adding Antibody - 1 and keeping one night in 4 X,, Antibody -2 for 30 minutes in wet box in 37°C and then adding SP compounds for 30 minutes, DAB coloration under the control of the observation of microscope, staining hematoxylin after that, deaquating, transparence and then mounting u-sing neutral GUM. The method of positive result of VEGF and PTEN was defined as followings: selecting 10 high scope (400 x ) randomly, the number of positive cell of each scope ^ 5, positive ( + ) . positive cells contrasted to all cells ^30% ,positive ( + ) . Positive cell >30% and <50% , more positive ( + + ). Positive cell ^ 50% , most positive ( + + +). We combined grade I and grade II of glioma to low grade glioma( LGG) ,and grade III and grade IV to high grade glioma( HGG). We used X2 — test and Fisher' s exact probabilities in 2 x 2 table as statistic test.Result1. VEGF: With the increasing of pathological grades of glioma,the positive rate of VEGF was 15.38% for grade 1, 33.33% for grade II, 70.00% for grade III, 87.50% for grade IV. And there was significant deviation between LGG (I+ 11) andHGG(III+IV)(P<0.05).2. PTEN: With the increasing of pathological grades of glioma, the positive rate of PTEN was 53. 85% for grade I, 46.67% for grade II, 30.00% for grade III, 0.00% for grade IV. And there was significant deviation between LGG (I + II) andHGG(III+IV)(P<0.05).3. there was no dependability between VEGF and PTEN(P >0. 05).Conclusion1. Positive rate of VEGF was rising with the pathological grades of glioma. The expression of VEGF had closely relationshiop with the pathological grades ofglioma.2. Poorly differentiated glioma had defect of PTEN . Positive rate of PTEN was decreasing with the rising of pathological grades of glioma. The expression of PTEN had closely relationshiop with the pathological grades of glioma.