| Objective:Lung carcinoma is one of the common malignant tumor in the world.The incidence and mortality of lung cancer has shown a clear upward trend.Non-small cell lung cancer(NSCLC) comprises about 80% of lung cancers diagnosed.As the incidence of hidden and the performance of non-specific symptoms,lung cancer does not attract attention and timely treatment in patients and results in illness delay.Treatment often has come in late,which severely affects the patient's survival rate and quality of life.The 5-years survival rate of all stages lung cancer is only 15%,and this situation has not improved for 40 years.Metastasis of carcinoma cell is an important factor of leading to patient's death.These complex processes contain detachment from primarily focus,invasion into perienchyma,incycle of circulation system and lymphatic system,metabasis of distant organ and proliferation of new metabasis.The mechanism of invasion and metabasis of lung carcinoma are not yet understood.Therefore,the establishment of early diagnosis and prompt prognosis of patients with molecular markers become the focus of the study.Molecular biology and tumor formation mechanism of progress of the study may identify potential molecular targets and the development of new targeted therapy.PTEN is the only dual phosphatase activity of tumor suppressor gene, mainly by making the second messenger PIP3 inhibition of PI3K/Akt de-phosphorylation signal transduction pathway and plays a key role in the process of in apoptosis, cell cycle arrest, cell migration.VEGF is considered the most important angiogenesis-promoting factor,which can combine specific vascular endothelial cells and promote vascular endothelial cell proliferation, increase vascular permeability to macromolecules and tumor cells through blood vessels into the blood circulation. VEGF secretion of some cytokines may promote tumor cell proliferation,and make the tumor never-ending growth. VEGF is also associated with tumor growth, metastasis and prognos.Microvessel density test is to use a number of vascular endothelial cell-specific antigen for the antibody (eg, CD34) immune markers of vascular endothelial cells, and then counted under high-power microscope field of vision the number of capillaries, as the evaluation of an indicator of angiogenesis.The tumor growth and metastasis of Non-small cell lung cancer and other biological characteristics of solid tumors depend on angiogenesis.Angiogenesis is essential for tumor growth and metastasis. Tumor growth of more than 1-2 mm3 size, depends heavily on angiogenesis. MVD is considered the best response to tumor angiogenesis indicators. Most studies suggest that high MVD values are indicators of poor prognosis.In recent years, with regard to NSCLC in PTEN, VEGF, MVD of the study, some experimental results are quite different. We regard lung carcinoma as study object now, to explore the expression of PTEN, VEGF, MVD protein in lung carcinoma and adjacent non-neoplastic tissues, to explore their role in the process of tumorigenesis, progression, invasion and metastasis of lung carcinoma and their correlation, to clarify the biological behavior of lung cancinoma and the clinical relevance, to provide a theoretical basis for the occurrence and development of NSCLC and treatment.Methods:Immunohistochemistry was employed to measure the expression of PTEN, VEGF, MVD in 60 specimens of lung carcinoma tissue and 60 specimens of adjacent non-neoplastic tissue. SPSS 13.0 was applied to analyze the results of experiment.Results:1 Expression of PTEN and relation with clinical parameterImmunohistochemistry was employed to measure the expression of PTEN in 60 specimens of lung carcinoma tissue and 60 specimens of adjacent non-neoplastic tissue. The positive expression rate of PTEN protein in lung carcinoma tissues was 48.3% (29/60),while that in the adjacent non-neoplastic tissues was 88.3%(53/60). There was significant difference in the positive rates of PTEN protein between lung carcinoma tissues and the adjacent non-neoplastic tissues (P<0.05).Well-differentiated and moderately differentiated group of PTEN with no lymph node metastasis positive expression rate was significantly higher than poorly differentiated group with lymph node metastasis group, significant difference (P<0.05). Expression of PTEN wasn't correlated with sex,age, pathological grading of tumor and TNM (P>0.05).2 Expression of VEGF and relation with clinical parameterImmunohistochemistry was employed to measure the expression of E-cadherin in 60 specimens of lung carcinoma tissue and 60 specimens of adjacent non-neoplastic tissue. The positive expression rate of VEGF protein in lung carcinoma tissues was 66.7% (40/60) , while that in the adjacent non-neoplastic tissues was 16.7%(10/60). There was significant difference in the positive rates of VEGF protein between lung carcinoma tissues and the adjacent non-neoplastic tissues (P<0.05).Well-differentiated and moderately differentiated group of VEGF, no lymph node metastasis positive expression rate was significantly lower than poorly differentiated group with lymph node metastasis group, significant difference(P<0.05). Expression of PTEN wasn't correlated with sex,age, pathological grading of tumor and TNM (P>0.05).3 Expression of MVD and relation with clinical parameterImmunohistochemistry was employed to measure the expression of MVD in 60 specimens of lung carcinoma tissue and 60 specimens of adjacent non-neoplastic tissue. MVD values were 9.563±4.012 and 2.689±1.321, the former was significantly higher than the latter, there is statistical significance.Expression of MVD wasn't correlated with sex,age,cell differentiation, lymph node metastasis, pathological type, TNM staging(P>0.05).4 At the protein level, there were negative correlations between PTEN and VEGF expression (r=-0.519, P<0.05);MVD values of PTEN expression of positive and negative carcinoma were 7.014±3.425 and 9.915±4.176,the latter than the former,but no statistical significance(P>0.05). MVD values of VEGF expression of positive and negative carcinoma were 10.820±4.352 and 7.241±3.493,the former than the latter,but no statistical significance(P>0.05).Conclusions1 The higher expression of VEGF and the lower expression of PTEN were correlated with tumorigenesis and progression of NSCLC.2 The higher expression of VEGF and the lower expression of PTEN were correlated with malignant biological behavior of NSCLC, which included lymph node metastasise and pathological grading of the tumor.3 PTEN and VEGF maybe collectively participated in a series of pathological progression (eg: tumorigenesis, progression, invasion and metastasis) of NSCLC.4 PTEN, VEGF and MVD are helpful to evaluate the prognosis of NSCLC. It may find markers and targets for the early diagnosis of NSCLC and provide new idea and therapy for clinical treatment of NSCLC。... |
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