| In this study, puerarin, astragalus polysaccharide(APS), total flavone of tartarian buckwheat(TFTB) were extracted from different herbal medicine. Hyperlipidemia rat modals were induced by high-lipid-diet. Simultaneity,other thirteen groups rats were fed with high lipid diet supplemented with different concentration of astragalus polysaccharide, total flavone of tartarian buckwheat, puerarin-L-arginine composition , respectively.The experiment last three mouthes. After the experiment, the blood lipid level, lipid deposition in liver, antioxidant ability of serum and liver tissue, liver and other organ's histological changes in rat with hyperlipidemia were observed to study their regulation effect on lipid metabolism and antioxidant ability. The regulation effect on lipid metabolism and antioxidant ability of active components or composition from different herbal medicine were compared to screen side effect free substance with high efficiency of regulation effect on lipid metabolism. The results show as follows:1. Hyperlipidemia rat model was induced successfully by high-lipid-diet.Compared with normal control group, in high lipid model group serum Ch, Tg,LDL-C significantly increased 18.4%, 38.9%, 29.8%, serum HDL-C decreased 16.7%(P<0.01);Liver Ch , Tg concentration and liver index increased 500%, 88.6% and 25.3%(P<0.01), respectively.2. LPZ significantly reduce serum Ch , Tg, LDL-C concentration and serum oxidative stress in rat with Hyperlipidemia, but has no significantly influence on lipid deposition in liver and has a trend to raise oxidative stress in liver.Compared with high lipid model group, in Lipingzhi capsule group, serum Ch, Tg, LDL-C significantly decreased 23.7%, 26.1%, 25.2%(P<0.01);Liver Ch , Tg concentration decreased 19.4%, 10.6%, but there were no significantly statistical differences (P>0.05).3.The lipid regulation and antioxidant ability of TFTB from different parts of tartarian buckwheat is different. TBSE possess superior function than the other. TBSE not only significantly decrease serum Ch, Tg, LDL-C level, reduce lipid deposition in liver and reduce serum and liver oxidative stress in rat with hyperlipidemia, but also has a trend to raise the serum HDL-C level.3.1 Compared with high lipid model group, in H and L dose TBBE groups serum Ch decreased 7.9%(P<0.01), 1.3%(P>0.05), Tg decreased 11.1%(P<0.01), 1.8%(P>0.05), LDL-C decreased 13.7%(P<0.01), 9.9% (P<0.01);In H and L dose TBSE groups serum Ch decreased 21.9%(P<0.01), 19.7%(P<0.01), Tg decreased 30.5%(P<0.01), 19.9%(P<0.01), LDL-C decreased 19.1%(P<0.01), 16.8%(P<0.01);in H dose tartarian buckwheat haulm and flower groups Ch decreased 18.9%(T<0.01), -0.4%(P>0.05), Tg decreased U.\%(P<0.0\), 7.5%(P<0.05), LDL-C decreased 18.3%(/)<0.01), \.5%(P>0.05).3.2 Compared with high lipid model group, in H and L dose TBBE groups liver Ch decreased 47.2%(iJ<0.01), 36.1%(P<0.05), Tg decreased samely 9.1%(P>0.05);In H and L dose TBSE groups liver Ch decreased 55.6%(P<0.01), 44.4%(P<0.01), Tg decreased 16.7%(P<0.01), 7.6% (P>0.05);In H dose tartarian buckwheat haulm and flower groups Ch decreased 41.7 % (PO.01), 13.9%(P>0.05), Tg decreased 15.2%(P<0.01), 7.6% (?>0.05) .4. APS dose-dependentedly decrease serum Ch, LDL-C level, reduce lipid deposition in liver and reduce serum and liver oxidative stress in rat with Hyperlipidemia, but has no significantly influence on serum Tg .Compared with high lipid model group, in H, M, L dose of APS groups, serum Ch significantly decreased(/?<0.01) 18.8%, 17.4%, 14.2%, LDL-C significantly decreased(PO.Ol) 26.7%, 25.2%, 21.4%, Tg and HDL-C have no significantly statistical changes;Liver Ch significantly decreased(T<0.01) 58.3%, 25.0%, 30.6%, Tg decreased 25.8%(P<0.01), 10.6% (P>0.05) ,3.0% (P>0.05) .5. Puerarin-L-arginine composition dose-dependentedly decrease serum Ch, Tg, LDL-C level, reduce lipid deposition in liver and serum , liver oxidative stress in rat with hyperlipidemia.Compared with high lipid model group, in H, M, L dose of puerarin-L-arginine composition groups, serum Ch significantly decreased(P<0.01) 22.8%, 16.7%, 14.9%, Tg significantly decreased(P<0.01) 29.6%, 27.4%, 17.2%, LDL-C significantly decreased(P<0.01) 26.0%, 24.4%, 16.8%, and HDL-C have no significantly statistical changes;Liver Ch significantly decreased(P<0.01) 55.6%, 36.1%, 36.1%, Tg decreased 18.2% (PO.01), 7.6% (/>>0.05) ,4.5% (P>0.05) .In summary, through this series studies, it show that all the TBSE, APS and puerarin-L-arginine composition have lipid regulation and antioxidant ability. TBSE possess superior function than the other. TBSE not only significantly decrease serum Ch, Tg, LDL-C level, reduce lipid deposition in liver and reduce serum and liver oxidative stress in rat with hyperlipidemia, but also has a trend to raise the serum HDL-C level. Even has superior liver protective effect than LPZ. This result could provide theory support for exploitation natural lipid regulation medicine.
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