Protective Effects Of Polypeptides From Chlamys Farreri On Murine Damaged By ～(60)Co?-ray

Objective To investigate the anti-radiation effect of polypeptide from Chlamys farreri (PCF) on mouse radiated by ⁶⁰Co gamma ray in vivo. Methods We established the radiation damage models of Kunming mice by ⁶⁰Co gamma ray (6.0 Gy, at a dose rate of 0.65Gy/min) in vivo. The animals were randomly divided into six groups, including control group and five ⁶⁰Co groups (model, low dose, middle dose, high dose polypeptide from Chlamys farreri (PCF) and nilestriol positive control). We calculated the spleen and thymus indices, counted the white cells of the animals. And we measured the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), malondialdehyde (MDA), reactive oxygen species (ROS) and total antioxidative capacity (T-AOC) of the animals. Furthermore we also measured the formation of micronuclei and the mutation of chromosomes in mice. Results the results indicated that at the dose of 6.0 Gy, polypeptide from Chlamys farreri (PCF) could increase the spleen and thymus indices, inhibit the decrease of SOD, GSH-px activities and T-AOC and the increase of the amount of MDA and ROS of several organs in mice with dose dependent manner. But polypeptide from Chlamys farreri (PCF) had no influence on the amount of white cells, the formation of micronuclei and the mutation of chromosomes. Conclusion PCF had protective effects on mouse irradiated by ⁶⁰Co in vivo. The mechanisms might be attributed to its antioxidant capability.