Protective Effect Of Polypeptides From Chlamys Farreri On Murine Thymocytes Damaged By ～(60)Co γ-ray

Obsjective To study the effect and mechanism of polypeptide from Chlamys farreri(PCF) on the thymocytes damaged by ~60Co γ-ray(3650uJ/ cm2).Methods The cells were randomly divided into six groups: control group, ~60Co groups (model, 0.5% PCF, 0.25% PCF, 0.125% PCF, 0.1%VitC). The concentration of GSH-Px,ROS,A-SAC,T-AOC and the cells' viability were determined by biochemical and MTT methods respectively. The mitochondria memberine potential were tested using flow cytometry (FCM). The expressions of P53, Bax and Bcl-2 proteins were examined by immunocytochemical technica.Results The results indicated that PCF could enhance the activities of GSH-Px, A-SAC, T-AOC and OD values in cells. PCF decreased the amounts of ROS. It also raised the expression of Bcl-2 gene and decreased the expression of P53, Bax. The results showed that the observed indexes of the PCF groups are significantly different compared with model group (P<0.05).Conclusions PCF has the protective effects on damages of thymocytes caused by the irradiation of ~60Co. The mechanisms might be related to inhibiting of the amount of ROS and raising the expression of Bcl-2 gene, increasing anti-oxidative enzymes in cells and inhibiting the lipid peroxide.