| Objective: The safety and myocardial protective effect of prolonged perfused ventricular fibrillation under moderate hypothermia were investigated.Methods: Twenty-four Wistar rats were randomly divided into two groups. Isolated rat hearts were subjected to preperfusion for 15 min, then experiment group (n=12 ) under perfused ventricular fibrillation with moderate hypothermia and continuous venting maintained for 120 min; control group (n=12) received ischemia for 120 min, during ischemia, every 30 min, St. Thomas' Ⅱ solution was intermittent perfused for 3 min. Then, both groups were reperfused with modified Krebs-Henseleit buffer at 37℃ for 40 min. Then, coronary flow was monitored. Myocardial enzyme creatine kinase-MB (CK-MB), cardial troponin I (cTn-I) were detected. Myocardial adenosine triphosphate (ATP) level and myocardial water contents were measured. Myocardial ultrastructure was observed.Results: The improvement of myocardial ultrastructure and coronary flow inexperiment group were better than those of control group. Myocardial enzymeCK-MB , cTn-I in experiment group were lower than those in control group(P<0.05) ,myocardial ATP level were significantly higher than those in control group(P<0.01) .Conclusion: Prolonged vented fibrillating hearts under moderate hypothermia with giving continuous adequately perfused might be a safe modality to employ and was superior to that of traditional St. Thomas' Ⅱ solution in myocardial protection.
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