Anti-coxackievirus B3 Action Of Several Medicinal Plant Extracts In Vitro

Objective: Evaluate the antiviral activity of of Potentilla freyniana, Ampelopsis sinica, A. humulifolia var.heterophylla and Selaginellalabordei on coxsackievirus B₃ in vitro..Then evaluating the anti-coxsackievirus activity of the four effective extracts of Selaginellalabordei and the compounds of two effective extracts of Ampelopsis sinica.Methods: In these experiments, Antiviral activity was investigated by observing cytopathic effect (CPE), by using MTT colorimetric assay and Crystal violet Asaay for viable cell rate in Hep-2 cells. And TI,TC₅₀,EC₅₀, TC₅₀,EC₅₀,SM was utilized as criterion to assess the effects of the drugs. Virazole was used as positive control.Results: (1) The TC₅₀ of four extracts were 7.2, 8.1, 8.7, 12.4 μg-mL^-1 respectively. Potentilla freyniana, Ampelopsis sinica and Selaginella labordei showed the abilities of directly-killing viruse and the EC50 were 0.52,1.21,0.53μgmL^-1 respectively. So the TI were relatively figured out as 14,6.7,23. Ampelopsis sinica and Selaginella labordei showed the ability of directly-antiviral and the IC50 were 0.56,0.5 lμg-mL^-1. So the TI were 16 and 23 respectively. The extracts had not showed abilities of inhibiting viral biosynthesis. (2) The TC50 of four extracts of Selaginella labordei were 61.41,52.94,25.34,74.17μg-mL^-1 respectively. A,B,C showed the ability of directly-antiviral and the EC₅₀ were 10.89,3.72,4.07μg·mL^-1. So the TI were 5.64,14.03 and 6.23 respectively. A,B,C,D showed the abilities of directly-killing viruse and the EC₅₀ were 2.46,1.02,0.71 and 1.69μg·mL^-1 respectively. So the TI were figured out as 24.96,51.90,35.69,43.89 and the SM were 2.20,5.81,4.62,5.60 relatively. C showed abilities of inhibiting viral biosynthesis, The EC₅₀ were 5.08 and 5.88μg·mL^-1 , TI were 10.42 and 4.32. (3) The TC₅₀ of 1^#5# compounds of Ampelopsis sinica were 4.24, 4.32, 5.48, 9.37, 9.37 μg·mL^-1 respectively. They had not showed the abilities of directly-antiviral. 3^*,4^#,5^# showed the abilities of directly-killing viruse and the EC50 were 0.40,0.82 and 0.52μg·mL^-1 respectively. So the TI were figured out as 13.7,11.4 and 18.0 relatively. 4^#,5^#showed abilities of inhibiting viral biosynthesis, The EC50 were 0.46 and 0.40μg·mL^-1, TI were 20.4 and 23.4.Conclusions: (1) The four drugs had the action of anti-CVB3 in several ways. (2) The four ectracts of had good action of anti-CVB3.A,D showed the abilities of directly-killing viruse. B and C showed abilities of inhibiting viral biosynthesis , directly-antiviral and directly-killing viruse . (3) The Cytotoxity of M and N were gravely and action of anti-CVB3 were fainty, but the cytotoxity become less and showed abilities of inhibiting viral biosynthesi and directly-killing viruse after they were compounded. (4) By using MTT colorimetric assay and Crystal violet Asaay for viable cell rate,we can evalue the anti-coxsackievirus activity more comprehensive.