The Experimental Study On Restraining Gastric Carcinoma' Occurrence And Development By Celecoxib

[Objective]The investigation measure the expressions of COX-2(cycloxygenase-2) in gastric carcinoma and carcinoma side tissue, survey the expressions of cell increment and VEGF(vascular endothelial growth factor), with the intervention of celecoxib (COX-2 inhibitor), and make nude mouse transplantation tumor model experiment, in order to explore the relationship of cycloxygenase-2, cycloxygenase-2 inhibitor to gastric carcinoma.[Methods]1. Detect the expression of COX-2 in gastric carcinoma and carcinoma side tissue (to classify gastric carcinoma by three standardization: the state of lymph node metastasis, clinical pathology staging: morning stage, intermediate stage, advanced stage, and tissue pathology staging: cell differentiation fine, cell differentiation moderately, cell differentiation inferior ) , through SP immunohistochemical method.2. Apply cell counting to detect the effect of celecoxib to the growth curve of gastric carcinoma cell SGC7901 and BGC-823. Gastric carcinoma cell SGC7901 and BGC-823 are conventionally cultured in RPMI-1640 medium, containing 10% bovine calf serum, and take count of two pieces of 24 hole plank every day according to plan, keeping eight days. Draw cell growth curve after different drug concentration intervening, take cultivate time as abscissa, and take cell logarithm as ordinate.3. Apply MTT chromatometry to detect the influence of celecoxib on the survival rate of the cells SGC7901 and BGC-823. Gastric cancer cells SGC7901and BGC-823 are conventionally cultured by RPMI-1640 nutrient medium, containing 10% Bovine Calf Serum. The survival of the control cell group is noted by 100%. The survival of experimental cell group iscalculated according to the following formular: survival rate = OD value of experimental group / OD value of control group × 100% .4. Apply ELISA ( enzyme-linked immunosorbent assay) to detect the influence of celecoxib on exudation of VEGF protein of gastric carcinoma cell SGC7901 and BGC823. Gastric carcinoma SGC7901, BGC823 are conventionally cultured in RPMI-1640 medium, containing 10% bovine calf serum, and add drug after the cell adhere to wall. Set up dose-effect relation group and time-effect relation group. End experiment after four days, and extract cell supernate. According to ELISA reagent box, build up VEGF protein standard curve, detect OD value in 450nm, and educe VEGF protein concentration through the curve.5. Nude mouse transplantation tumor experiment under celecoxib intervening. AnabiosisSGC7901, BGC823 cell plant, go down to posterity, and choose the fine growth state cell, subcutaneous inoculate in nude mouse oxter. Kill nude mouse after thirty days, weigh tumour tubercle, restrain tumour rate according to the following formular: restrain tumour rate ( % ) = (average weigh of contrast group tumour—average weigh of experimental group tumour) / average weigh of contrast group tumour * 100 calculate restrain tumour rate.6. Statistics method adopt x2 test and t test. Data is expressed with mean±standard deviation, P < 0.05 express statistics difference notable.[Result]1. The expression of COX-2 occur in marked different betwent gastric carcinoma (to classify gastric carcinoma by three standardization: the state of lymph node metastasis, clinical pathology staging: morning stage, intermediate stage, advanced stage, and tissue pathology staging: cell differentiation fine, cell differentiation moderately, cell differentiation inferior) and paracancer tissue.2. Celecoxib has inhibitor effect to gastric carcinoma cell SGC7901, BGC823, and presents dose dependence and time dependence. The inhibitor effect to cell differentiation moderately of SGC7901 cell is feebler than to cell differentiation inferior of BGC823 cell.3. Celecoxib has inhibitor effect to exudation of VEGF protein of gastric carcinoma cell SGC7901 and BGC823. With the increasement of drug concentration and the prolonging of effect time, the exudation amount of VEGF also corresponding reduces.4. Celecoxib has inhibitor effect to the tumour growth of two kinds of nude mouse animal model in different degree. The inhibitor effect express dose and time dependence, and restrain tumour rate of BGC823 is higher than SGC7901, but difference isn't notability ( P > 0.1 ) .[Conclusion!The expression of COX-2 in gastric carcinoma tissue is higher than in paracancer tissue, and celecoxib can restrain the occurrence and development of gastric carcinoma tissue.