Investigation Of IL-2, SIL-2, TNFα And NK Cell Activity In Patients With Primary Hepatic Carcinoma

Objective: To investigate the levels of IL-2,sIL-2R,TNF α and NKcell activity,to discuss the causes of insufficience of cellular immunity function in patients with primary hepatic carcinoma and influence of these factors on patients.Methods: (1) The levels of IL-2, NK activity in PBMC were measured in 30 patients with primary hepatic carcinoma by MTT colorize assay. Be due to the ability of the absorbing MTT for the living cell, the activated xianliti could make a color reaction by dissolve the ring of MTT. MTT is a yellow dissovled material , after being oxgenized, it formed into the blue crystall, the later would be dissovled by isopropanol to make color. According to the level of the color reaction, we could understand the level of the cell metalism . After adding 10ul MTT into the every CTLL well and being incubated for 4 hours at 37℃ circumstance, add isopropanol right now after being taked out and mixed thoughly in order to dissove the deep blue crystal. It could be measured by the 570nm enzymed instrument after 10minnites at the room temperature. So we could get the activity of I L — 2 and NK cell by putting the measured OD value into the comper.(2) The levels of sIL-2R, TNFα in PBMC in 30 patients with primary hepatic carcinoma were measured by an enzyme linked immunosorbent assay (ELISA) . The basic mechnism of ELISA is: making antigen or antibody be combined with the surface of a solid carrier and maintain its immue activity;making antigen or antibody be combined with a enzyme into enzyme- antigen -antibody complex, it not only maintains antigen or antibody immue activity , but also maintains enzyme activity. When measuring, make samples and antigen or antibody labled enzyme becombined respectly with antibody or antigen on the surface of the carrier, then separating antigen or antibody complex from the other matetials by washing methods. Finally, the quantility of the enzyme on the surface of the carrier is in proportion to the quantility of antigen or antibody in the samples. After adding the substrates and being translated into the color products, the quantility of the products is directly relation to the quantility of the samples, so we could make the quantility or quatility analysis according to the deepness of the color reactionsC2].Results: The levels of IL-2and TNF a were significantly lower in primary hepatic carcinoma than control group (the both assayed P<0.01);The levels of NK activity were significantly lower in primary hepatic carcinoma than control group ( P<0.05);but he levels of sIL-2R were significantly higher than in control group (P<0.05) .Conclusion: The levels of IL-2> TNF a and NK activity are decrease in patients with primary hepatic carcinoma. It is probably the causes for insufficient celluar immunity, it also helpful for us to understand the functions of celluar immunity and the curements of the clinic doctors for the patierts[3].