Anti-proliferation And Apoptosis Induction In Hepatocellular Carcinoma Cell By Celecoxib

AimTo study Anti - proliferation and apoptosis induction in hepatocellular carcinoma cell by Cyclooxygenase -2 (COX -2) inhibitor, celecoxib. And to explore their possible mechanism.MethodsDifferent concentrations of celecobix have been used in the culture of hepatocellular carcinoma cell bel - 7402 , hepG2, The number of cells was observed by means of MTT assay. Tumor cell apoptosis and cell cycle were studied by flow cytometry. Morphological changes were observed under stage - contrast microscope and HE stain, the expression of bcl -2,caspase -3 was assayed by immunohistochemical stain.Results(1) Compared with control group, the cell numbers were decreaseing significantly with the increase of celecoxib concentration and exposure time. Treated with celecoxib after 24h, 36h and 48h, The inhibitory IC₅₀ in bel -7402, hepG2 cell were 220. 3μmol/L,120. 8μmol/L, 92. 5μmol/L and 94. 2μmol/L, 78. 3μmol/L,51. 3 μmol/L respectively. and the r(correlation coefficient) values were 0.998,0.96,0. 89 and 0.968,0.94,0.99 respectively.(2) The number of apoptosis cell significantly increased with increasingdose of celecoxib.(3) After exposure to different concentrations of celecoxib for 48h, bel -7402 ^ hepG2 cells were accumulated in Gq/Gj phase while decreased in S phase.(4) After treated with celecoxib, the morphological changes of HepG2 and bel - 7402 cells that shrank and turned round, cytoplasmic condensed were observed through the stage - contrast microscope and HE stain.(5) When treated with celecoxib for48h,the expressions of bel -2 protein of bel - 7402N hepG2 cells were down - regulated, while the expressions of caspase - 3 protein were up - regulated.Conclusioncelecoxib could inhibit HepG2 and bel - 7402 proliferation through disturbing the cell cycle progression and inducing apoptosis, celecoxib treatment regulated the expressions of bel - 2 protein and caspase - 3 protein of bel - 7402 ^ hepG2 cells.