Expression Of COX-2,Ki67,VEGF In Laryngeal Squamous Cell Carcinoma And Its Significance

BACKGROUND:Laryngeal carcinoma which occupies 11%-22% ENT malignant tumor is the commonest one among ENT malignant tumor, Pathologically, 95%-99% laryngeal carcinoma is laryngeal squamous cell carcinoma. currently, the treatments and prognostic estimation are mainly based on TNM staging, the operations usually left the patients different extent functional impairment because its special place, so the new diagnostic method and more and more effective preventative and therapeutic tools based on molecular biology is emergency request.Presently, many studies showed that COX-2,Ki67,VEGF were highly expressed in various kinds of tumors. It may promote growth,proliferation and metastasis of tumor by many ways. In addition, there are probable correlations among them in the process of the growth, proliferation and metastasis of tumor. Although the pathogenesy of the tumor is not clear yet today, but the relation between the genes and the expressed products concerned with cell proliferation and angiopoiesis and tumor got more and more emphasis, so the effect of COX-2,Ki67,VEGF in the process of the tumorigeness and metastasis of laryngeal sqamous cell carcinoma is the new researching hot topic.OBJECTIVES:1 To examine the expression of COX-2,Ki67 and VEGF in laryngeal squamous cell carcinoma tissues,adjacent normal laryngeal tissues and vocal cords polyp tissues, and evaluate the expressive differences in the three different laryngeal tissues.2 To analyze the expression of COX-2,Ki67,VEGF in laryngeal squamous cell carcinoma tissues, and elucidate the effect of COX-2,Ki67,VEGF in the growth, proliferation and lymphatic metastasis of laryngeal squamous cell carcinoma and its mechanisms.3 To analyze the correlations among the expression of COX-2,Ki67 and VEGF in the development of laryngeal squamous cell carcinoma.MATERIAL AND METHODS:1. To collect 57 pathological specimens of laryngeal squamous cell carcinoma which belong to patients who accepted surgical treatment in The First Affiliated Hospital of Zhengzhou University from January, 2003 to December, 2004; simultaneously, 7 cases of adjacent normal laryngeal tissues; 9 cases of vocal cords polyps tissues in 2006.2. To investigate the expression of COX-2,Ki67 and VEGF in the three differentlaryngeal tissues by the way of immunohistochemistry.3. To evaluate the relations between the expression of COX-2,Ki67,VEGF and lymphatic metastasis, pathological grading and T staging. To evaluate the correlations among the expression of COX-2,Ki67 and VEGF in the development of laryngeal squamous cell carcinoma.RESULT:1 The expression of COX-2 protein was localized in the cytoplasm. The positive rates were 59.64%(34/57) in laryngeal squamous cell carcinoma tissues, 0(0/7) in adjacent normal laryngeal tissues, 0(0/9) in vocal cords polyps tissues. There were statistically significant differences among the expression of COX-2 in the three different laryngeal tissues (P <0.01); The expression of COX-2 had significant relations with lymphatic metastasis and T staging (P <0.01).2 The expression of Ki67 protein was localized in the nuclear. The positive rates were 68.42%(39/57) in laryngeal squamous cell carcinoma tissues, 14.28%(l/7) in adjacent normal laryngeal tissues, 22.22%(2/9) in vocal cords polyps tissues. There were statistically significant differences among the expression of Ki67 in the three different laryngeal tissues (P <0.05); The expression of Ki67 had significant relations with lymphatic metastasis, pathological grading and T staging (P <0.05).3 The expression of VEGF protein was localized in the cytoplasm. The positive rates were 75.43%(43/57) in laryngeal squamous cell carcinoma tissues, 28.57%(2/7) in adjacent normal laryngeal tissues, 33.33%(3/9) in vocal cords polyps tissues, There were statistically significant differences among the expression of VEGF in the three different laryngeal tissues (P <0.05). The expression of VEGF had significant relations with lymphatic metastasis, pathological grading and T staging (P <0.05).4 There were significantly positive correlations among the expression of COX-2,Ki67 and VEGF in laryngeal squamous cell carcinoma tissues (P <0.05).CONCLUSION:1 the high expression of COX-2,Ki67 and VEGF in laryngeal squamous cell carcinoma tissues may promote the growth, proliferation and metastasis of laryngeal squamous cell carcinoma.2 There was significantly positive correlation between the expression of COX-2 and the expression of Ki67 in laryngeal squamous cell carcinoma tissues, COX-2 may promote vascular growth, speed up the rapid proliferation of laryngeal squamous cell carcinoma tissues.3 There was significantly positive correlation between the expression of COX-2 and the expression of VEGF in laryngeal squamous cell carcinoma tissues, COX-2 may promote vascular growth by increasing expression of VEGF and provide excellent conditions for the growth, proliferation and metastasis of laryngeal squamous cell carcinoma.4 There was significantly positive correlation between the expression of Ki67 and the expression of VEGF in laryngeal squamous cell carcinoma tissues, Ki67 and VEGF have coordination in the occurrence and development of laryngeal squamous cell carcinoma, The proliferation of carcinomatous cells need the nutritious support of newly born vascular, In return, the proliferative carcinomatous cells promote vascular growth by autocrining VEGF.5 The simultaneously detecting of expression of COX-2,Ki67 and VEGF in laryngeal squamous cell carcinoma tissues have certain value for clinical appraisal; To inhibit the expression of COX-2,Ki67 and VEGF in laryngeal squamous cell carcinoma tissues may become new ways to control the growth, proliferation and metastasis of laryngeal squamous cell carcinoma.