Effect Of Sweet-potato Anthocyanins On Oxidation Condition In Aging Rats And Cytotoxicity Of PC-12 Cell Induced By Amyloid β Protein

AIM to investigate the effect of sweet-potato anthocyanins on oxidation conditions in aging mice and cytotoxicity of PC-12 cells induced by Amyloid β protein (A β). METHODS we use the natural aging Wistar rats as experimental models. The animals were randomly divided into six groups, including normal young control group, aging model group, low concentration, middle concentration and high concentration sweet-potato anthocyanins groups and vitmin E as positive control group. We measured the total antioxidative capacity (T-AOC), activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), reactive oxygen species (ROS) and malondialdehyde (MDA) of different organs from the animals. And we also established the cytotoxicity of PC-12 cell model induced by A β. By using cell culture, MTT assays, flow cytometry, we measured the cell viability, apoptosis, activity of caspase-3, and mitochondria membrane potential, intracellular Ca²⁺ in PC12 cells. RESULTS sweet-potato anthocyanins can increase the activities of T-AOC, SOD and GSH-px and decrease the amount of ROS and MDA in several organs from the animal in a dose-dependent manner (p<0.05, p<0.01). MTT assays showed that the PC-12 cell viability decreased under A p treatment. Sweet-potato anthocyanins can increase the PC-12 cell viability, stabilize the mitochondria membrane potential, and decrease the concentration of intracellular Ca²⁺ dose dependently (p<0.05, p<0.01). CONCLUSION sweet-potato anthocyanins modulate the oxidation condition of aging rats through its ability of compensating the decline in T-AOC and the activities of antioxidant enzymes and thereby inhibit the aging process. Also sweet-potato anthocyanins can protect the PC-12 cell from Aβ induced injury. Thus it may be used as a potential material in anti-neurotoxicity.