The Clinical Application Of Testing HBV DNA In Semen Of HBV Infected Patients With PCR-ELISA

OBJECTIVE1,To detect hepatitis B virus (HBV) in senem of HBV infected patients and study the relationship between the infective state of HBV in blood serum and the quantity of HBV DNA in semen. To find the evidence for HBV DNA vertical transmission of hepatitis B virus DNA by sperm.2,Compared with the value of the clinical application of Testing HBV DNA in semen with PCR-ELISA and FQ-PCR.MATERIALS AND METHODSThe 52 men with HBV infected patients were collected. They blood serum HbsAg are positive. Collected their sperm. PCR-ELISA was used to detect HBV DNA in semen and blood serum samples of 52 HbsAg seropositive patients. Amplification by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect semen samples of 52 HbsAg seropositive patients. To compare the results. Analysis the line of blood serum and semen HBV DNA copies of logarithm. Detect HBVDNA in semen and serum. And study the relationship between the infective state of HBV in serum and the quantity of HBV DNA in semen. To compare HBV DNA copies and positive of semen, Detect PCR-ELISA and FQ-PCR merit or demerit.RESULT1. PCR-ELISA was used to detect HBV DNA of 52 patients. The positive rate of semen with HBV infected patients HBV DNA was 15.38% (8/52). with 4.06×10~4 copies/㏕ on average. The positive rate of blood serum HBV DNA was 73.08% (38/52) with 2.05×10~7 copies/㏕ on average. There is positive rank correlation between semen HBV DNA copies and serum HBV DNA copies.The HBV DNA copies is lower in semen than serum.2. FQ-PCR was used to detect HBV DNA of 52 patients. The positive rate of semen with HBV infected patients HBV DNA was 19.23% (10/52) with 4.27×104 copies/㏕ on average.3.Chi-square test and wilcoxon signed-rank test was used to two methods detect HBV DNA in semen. The positive rate and copies of HBV DNA in semen were not statistically significant.CONCLUSION 1.Part of semen of HBV infected patients is infective.2.There is positive rank correlation between semen HBV DNA copies and serum HBV DNA copies.3. No significant difference in PCR-ELISA and FQ-PCR was used to detect HBV DNA in semen of HBV infected patients. But PCR-ELISA and FQ-PCR quantitative test methods has special features and very sensitive. They has important clinical significance for learning the situation of HBV DNA copies.