Expression And Significance Of Integrin β1 And MMP-2 In Adenomyosis

Adenomyosis is a gynaecological common discordor with benign uterinedisease in which ectopic endometrial glands and stroma grows in themyometrium.. more take menorrhagia,dysmenorrhea, infertility and uterineenlargement as the main clinical manifestations. Because this disease differentdiagnostic technologies and standards, different countries, races and hospitalsreported adenomyosis incidence vary between fluctuations in 10%~60%.Nevertheless, in recent years the literature has reported the incidence ofadenomyosis risen gradually, rising trend has been increasing attention tounderstanding the adenomyosis, study the underlying causes of morbidity andseek effective treatment programmes are particularly important.The pathogenesis of adenomyosis involved various many aspects , betweeneach kind of pathogenesis factor exists relates mutually and promotes mutually,but non-sole factor pathogenesis. The disease occurred the concrete mechanismcan not be clear about, but will not be able to leave the endometrium planter andthe growth adherency,the invade and the blood vessel which endometriosisrecognize produces "3A" the procedure, research integrin adhesion, MMPsdegradation in the basilar membrane with adenomyosis morbidity will help tofurther the understanding of etiological basis.The main components of ECM is collagen,laminin, fibronectin, integrinthat contains beta subunit can mostly integate these matix elements .Integrin(integrin, Int) is one kind cell surface adheres molecular, by alpha and beta twosubunit constitution, but alpha, beta subunit respectively has many types, Int β1is then one of them. Int β1 is the important beta subunit in the integrin moleculefamily, that can unify with the different subunit, constituting the vast majority ofECM receptors ,and is the cell and the intercellular space,in particular cell andECM indispensable adheres molecular. Integrin not only referred to theconglutination of different ECM components with cell material, and induce cellmultiplication ,differentiation,migration and express some ECM and MMPsgenes that induce cell to identify surrounding ECM components, resulting ininvade,multiplication process and so on play an important role. Adenomyosis inthe animal model studies show that Int β1 expression in the adenomyosisgroup apparent increase, and more usual for the macrophage secretion.The basilar membrane ingredient degeneration is that the ectopicendometrium can plant and grow in the myometrium, forms condition which theadenomyotic ectopic focus must have. Matrix metalloproteinase is a big kind ofzinc dependence endopeptidase enzyme family, may degrade one kind or severalkinds of ECM ingredient, gelatinase MMP-2 function substrate IV collagen isthe ECM principal constituent. At present some scholars have think the viewthat the ectopic focus express the activity of MMP-2, to make it possible invadearound organizations.Adenomyosis is one kind benign disease with malignant tumor invadebehavior, has enzyme degradation, migration, the multiplication in the matrixand so on with the tumor invade behavior similar a series of processesperformance.Integrin and the matrix metalloproteinase participate in this processthe occurrence. This experiment deterts the expression of Intβ1 and MMP-2 inthe eutopic and ectopic endometrium of adenomyosis group and endometrium ofcontrol group .by immunohistochemical SP method,for further studies theadenomyosis to provide the theory basis.Methods:by selecting during hysterectomy of adenmoyosis in-patients (30cases) as a experimental group, collect eutopic and ectopic endometrium tissuesof that (18 cases in the proliferative phase , and 12 cases in the secretivephase), by selecting during hysterectomy of uterine leiomyomas endometrium ascontrol group , 20 cases (10 cases in the proliferative phase , and 10 cases in thesecretive phase) .Immunohistochemical SP method was employed to detect theexpression of Intβ1 and MMP-2 in the eutopic and ectopic endometrium ofadenomyosis group and endometrium of control group .Results : the expression of Intβ1 was no difference between proliferativephase and secretory phase in eutopic endometrium of adenomyosis group(P>0.05), in control group that in the endometrium of secretory phase washigher than that in the endometrium of proliferative phase (P<0.05). Theexpression level of Intβ1 in ectopic endometrium of adenomyosis group wassignificantly higher than that of eutopic endometrium of adenomyosis(P<0.05)and endometrium in control group (P<0.05) .But no significantdifference between in eutopic endometrium of adenomyosis group andendometrium in control group was observed (P>0.05). The expression ofMMP-2 of secretory phase was higher than that of proliferative phase in eutopicendometrium of adenomyosis group (P<0.05). The staining intensity of MMP-2in the endometrium of control group of secretory phase is obviously higher thanproliferative phase (P<0.05). The expression level of MMP-2 in ectopicendometrium of adenomyosis group was significantly higher than that of eutopicendometrium of adenomyosis (P<0.05) and endometrium in control group(P<0.05) .But no significant difference between in eutopic endometrium ofadenomyosis group and endometrium in control group was observed (P>0.05).The expression of Int β1 and MMP-2 in endometrium of control grouppresented the periodic variation along with the ovarian hormone change, theexpression of both may be involved in hormone . Int β1 in eutopic endometriumexpress periodic variation change, accord with eutopic endometriumdeterminism, the eutopic endometrium of adenomyosis patient has uniquenesspossibly which to be taken bad;The expression of Intβ 1 in ectopicendometrium rised , for the intersititial cell gathered into conglobation, thusfurther stimulating the muscle cell excessive hyperplasia provided theprecondition. Under the function of the inflammation cell factor, themacrophage activates, then proceed to the next step forms the waterfall effect,releases Intβ 1 to lead cell adhesion, the migration, the multiplication and so on.The MMP-2 excessively expression causes endometrium aggressivity tostrengthen, the ectopic endometrium organization that the aggressivity is strongthan that of eutopic endometrium was able to degrade extracellular matrixingredient of the myometrium around the ectopic endometrium including basilarmembrane, destroyed which has prevented the endometrium invasion " naturalbarrier ", at the same time the inflammation factor also might induce themacrophage to secrete MMP-2. This group of experimental result showed, in theectopic endometrium group activeness of expression between MMP-2 and Intβ1possibly has the intrinsic link, both has provided the condition together for theadenomyosis ectopic focus of formation. Integrin can adjust MMPs theexpression and the activation, at the same time adhere to MMPs and ECMrespectively,make MMPs lead the target goal.Conclusion : the expression of Int β1 and MMP-2 in ectopic endometrium ofadenomyosis group was signification higher than eutopic endometrium ofadenomyosis and endometrium of control group, both of which might beprompted with adenomyosis related morbidity. Int β1 and MMP-2 inendometrium of control group between secretory phase and proliferative phaseexpress difference ,both in the endometrium of the cyclical changes may beregulated by ovarian hormone, regulate and control of endometrium form andthe function change .The expression of Int β1 in eutopic endometrium ofadenomyosis group lose the periodic variation, the proliferative phase in thatpresent the high expression tendency, but secretory phase in that reduces thetendency, prompts eutopic endometrium to have the latent hereditycharacteristic which adenomyosis is taken bad.