The Relationship Between GT Repeat Sequence Polymorphism Of Na-K-2Cl Cotransporter Gene And Essential Hypertension In Kazakans Of Xinjiang.

Objective: To evaluate the relationship between GT repeat sequence of Na-K-2Cl-cotransporter(NKCC2) gene and essential hypertension(EH) in Kanakas. Methods: A population-based case-control study was carried out. The study population was selected randomly from the pasturing area aged 30 to 55 years in Xinjiang province. The study cohort consisted of 303 subjects (152 hypertensive patients, 151 normotensive controls). Genomic DNA was conventionally extracted from the peripheral blood leukocytes by proteinase K digestion of nuclei. The GT-repeat polymorphism of NKCC2 gene was identified by polymerase chain reaction (PCR) with fluorescence labeled primers, capillary electrophoretic and automatic analysis Genetic Profiler for microsatellite Genescan. Allele was separated by fragment length. Results: There were 14 alleles ranging from 210bp to 238bp in all subjects. The allele frequencies were from 0.002 to 0.281, and allele A9 was the most frequent in the whole Kanakas population, the next, A5 and A8. The heterozygosity of GT repeat locus was 0.812, the polymorphic information content (PIC) 0.803. All of Allele frequencies of the two groups microsatellite DNA in Kanakas population were in Hardy-Weinberg equilibrium. There was no significant difference in distribution of GT repeat polymorphic genotype between EH and control groups (x2=16.885, p=0.205). Conclusion: The results suggest that GT repeat polymorphism of NKCC2 gene may not be associated with EH in Kanakas.