Effects Of Myocardial Ischemia-reperfusion And Formaldehyde On The Lung And Brain Peroxidation Injuries In Rats And The Protective Effects Of Glutathione

Objective: The aims of this study were 1. To investigate the early-stage in lung and brain injuries in rats which subjected to myocardial ischemia-reperfusion(MIR). To observe whether there were some protective effects on these injuries which subjected to ischemia preconditioning (IPC) and postconditioning 2. To compare with the protective effects of the glutathione(GSH) in different groups, during the first minute of reperfusion we use two different pathways of administration: left ventricular- aorta-coronary arteries and vein. 3. To study the effects of inhaling different amount of formaldehyde during myocardial ischemia-reperfusion on lung and brain injuries in rats . We investigated whether there were some synergistic effects on lung and brain injuries between formaldehyde and MIR , and observed the protective effects of GSH. Methods: In anesthetized open-chest rats, the left anterior descending artery (LAD) was occluded for 30min and reperfused for 3h. The pathway of left ventricular-aorta-coronary arteries was in one minute administrating of GSH(120mg/kg) through the cannula of monitoring pressure. To inhale different amount of formaldehyde during myocardial ischemia-reperfusion via trachea cannula .Results: 1.The effects of MIR on lung and brain injuries ( The changes of the concentration of malondialdehyde(MDA), the activity of superoxide dismutase(SOD)and glutathion peroxidase(GSH-Px)in the lung and brain tissues : Compared with group sham, group of MIR increased MDA concentration combined with decreasing of SOD and GSH-Px activity significantly in lung and brain tissue(p<0.05). Compared with group MIR, groups of IPC and postconditioning decreased MDA concentration combined with increasing of GSH-Px activity significantly in lung tissue(p<0.05). Compared with group MIR, groups of IPC and postconditioning decreased MDA concentration combined with increasing of SOD and GSH-Px activity significantly in brain tissue(p<0.05). 2.The protective effects of administering of GSH(120mg/kg) via left ventricular-aorta-coronary arteries comparison with via the routine vein on lung and brain injuries subjected to MIR. 2.1 The changes of the concentration of MDA, the activity of SOD and GSH-Px in the lung and brain tissues : 2.1.1 Compared with group MIR, via the left ventricular-aorta intervene group and via the vein group decreased MDA concentration combined with increasing of GSH-Px activity significantly in lung tissue(p<0.05) . 2.2.2 Compared with group MIR, via the left ventricular-aorta intervene group and via the vein group decreased MDA concentration combined with increasing of SOD and GSH-Px activity significantly in brain tissue(p<0.05). Via the left ventricular-aorta intervene group compared with via the vein group. The changes of group via the left ventricular-aorta intervene had higer SOD, lower MDA than group via the vein(p<0.05).