Study On The Postmortem Redistribution Of Difenidol Hydrochloride In Poisoned Rats

OBJECTIVE:1. To develop the postmortem distribution, postmortem redistribution and postmortem diffusion animal models of difenidol hydrochloride by an intragastric administration, observe mainly vital signs and pathology of animals after a fatal intragastric administration of difenidol hydrochloride. 2. To investigate the postmortem distribution and postmortem redistribution of difenidol hydrochloride in animals to provide a scientific evidence for the forensic identification of the poisoning death caused by difenidol hydrochloride. 3. To study postmortem diffusion in rabbits after an intragastric administration of difenidol hydrochloride to illustrate the possible mechanism of the postmortem redistribution. METHOD:1. Animal model and Collection of samples1.1 Postmortem distribution model: Twenty two rats were randomly allocated to two groups, the first group (six rats) were given an intragastric administration of 1600mg/kg weight of difenidol hydrochloride; the second group sixteen rats) were given an intragastric administration of 800mg/kg weight of difenidol hydrochloride. After having been observed for 4 hours, the rats, which were alive, were executed by a cervical vertebrae luxation. The rats were dissected as soon as the vital signs disappeared, and the specimens such as brain, heart, lung, liver, spleen, kidney, muscle (left leg), bile, urine, heart blood, peripheral blood, were sampled immediately.1.2 Postmortem redistribution model: Thirty rats were respectively given an intragastric administration of weight of 800mg/kg. After having been observed for 4 hours, the rats, which were alive, were executed by a cervical vertebrae luxation. As soon as the vital signs disappeared, the rats were randomly placed at room temperature. The specimens such as the brain ,heart, lung, liver, spleen, kidney, muscle (left leg) were collected at 0, 4, 8, 24, 48, 72 hours after the death and was analyzed in 24 hours.1.3 Postmortem diffusion model1.3.1 Postmortem diffusion: After having been shutted the windpipe completely for 12 minutes, twenty four domestic rabbits were respectively given an administration of difenidol hydrochloride (400mg/kg) by a stomach tube. The samples of every three rabbits were respectively and randomly collected at 1, 3, 6, 12, 24, 48, 72, 96 hours after the intragastric administration.1.3.2 The effect of the dose to the postmortem diffusion: Twelve domestic rabbits were randomly allocated to four groups, and given an administration of difenidol...