Librium Forensic Toxicology Dynamics Research

Objective1. To establish a chlordiazepoxide poisoning death model by intragastric administration, and observe mainly vital signs of rats and dogs.2. To establish a qualitative and quantitative analysis method by GC equipped with FPD and GC/MS for chlordiazepoxide in poisoning samples.3. To observe the postmortem distribution, postmortem redistribution and decompose kinetics in samples regular.Methods1. Postmortem distribution in chlordiazepoxide poisoning rats:the rats were randomly allocated to three groups, and respectively administrated chlordiazepoxide with0.5,2and4LD50by intragastric. They were autopsy after3h adminiatration, and collected heart, lung, liver, spleen, kidney, brain, heart-blood, muscle and testicle. The concentration of chlordiazepoxide was analyzed by GC and GC/MS.2. Postmortem redistribution in chlordiazepoxide poisoning rats:the rats were administrated chlordiazepoxide with0.5LD50by intragastric. They were autopsy after3h adminiatration, and collected heart, lung, liver, spleen, kidney, brain, heart-blood, muscle and testicle after death interval0,2,4,8,12,24,48,72,96h. The concentration of chlordiazepoxide in samples was analyzed by GC and GC/MS.3. Decompose kinetics in samples:the dogs were administrated chlordiazepoxide with4LD50by intragastric. They were autopsy after3h adminiatration, and collected blood, liver. Blood and liver were respectively allocated three parts, placed20℃、4℃、-20℃condition. The concentration of chlordiazepoxide in samples was analyzed after0、1w、3w、5w、7w、14w,5m,7m,16m.4. Statistics data was expressed as mean±SD model and analysed statistically with SPSS13.0software by One-Way ANOVA’s t-test.Results1. Poisoning symptoms:the animals were drowsiness after low dose exposure chlordiazepoxide, and showed coma after high dose exposure.2. Analysis method:the recovery, linear range and sensitivity corresponded the demand for analysis of poison in biological specimen, it can be used in the forensic identification and forensic toxicokinetics study of chlordiazepoxide poisoning death case.3. Postmortem distribution in chlordiazepoxide poisoning rats:the distribution of chlordiazepoxide in0.5.2and4LD50poisoning rats were no difference. 4. Postmortem redistribution in chlordiazepoxide poisoning rats:the postmortem redistribution phenomenon was observed in0.5LD50poisoning rats. The concentration of chlordiazepoxide in heart, liver, spleen, lung, kidney and muscle was influenced by death interval times. The concentration of chlordiazepoxide in brain and testicle were stabile.5. Decompose kinetics in samples:the concentration of chlordiazepoxide in blood and liver was decomposed with preserve time and influenced by conserve temperature. There was observed statistical significance meaning.Conclusion1. Chlordiazepoxide poisoning death model was established by intragastric administration. The model can be applied to the forensic identification of chlordiazepoxide poisoning death and forensic toxicokinetics.2. The qualitative and quantitative analysis method were established by GC equipped with FPD and GC/MS for chlordiazepoxide in poisoning samples, of which recovery, linear range and sensitivity meets the demand for analysis of poison in biological specimen, can be used in the forensic identification and forensic toxicokinetics.3. Postmortem distribution of chlordiazepoxide in poisoning rats:the distribution of chlordiazepoxide in0.5,2and4LD50poisoning rats were no difference. The concentration of lung was higher than other samples, which might be caused by an agonal breathing pattern.4. Postmortem redistribution of chlordiazepoxide in poisoning rats:the postmortem redistribution phenomenon was observed in0.5LD50poisoning rats.5. Decompositione kinetics of chlordiazepoxide in preserved samples:the concentration of chlordiazepoxide in blood and liver was decomposed with preserve time. Concentration of chlordiazepoxide in samples was influenced by conserve temperature.