Correlation Of E-cadherin Polymorphisms To Esophageal Squamous Cell Carcinoma And Gastric Cardiac Adenocarcinoma

Objective: E-cadherin(CDH1), as one of the cadherin members, relates with invasion and metastasis of various cancers. Polymorphisms in the promoter and untranslated region of E-cadherin gene have been associated with tumor deveploment and progression,via modifying its transcriptional activity and protein expression level. This study was designed to investigate the correlation of three single nucleotide polymorphisms (SNPs), -160C/A and -347G/GA in the promoter region and +54C/T in 3'untraslated region(UTR), to susceptibilities and lymphatic metastases of esophageal squamous cell carcinoma(ESCC) and gastric cardiac adenocarcinoma (GCA) in North China population .Methods: This hospital-based case-control study included 572 cancer patients (333 with ESCC and 239 with GCA) and 343 healthy controls. Genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure. CDH1 polymorphisms were analyzed by PCR-restriction fragment length polymorphism analysis (RFLP).Statistical analysis was performed using SPSS11.5 software package. P<0.05 was considered significant for all statistical...