The Effects Of Deoxynivalenol On The Proliferation Of Human Gastric Cells In Vitro

Objective: Deoxynivalenol (DON, vomitoxin) is a kind of Trichthecene mycotoxin, which is toxic meTableolite of Fusarium graminearum. DON contamination of grains and stuffs is a very common phenomenon world widely, and is one of predominating contaminating mycotoxins in the high incidence area of esophageal and gastric cancer in our country. DON could inhibit the cell mitosis, the synthesis of DNA and RNA, and the synthesis of biological macromolecules such as proteins, which showed that DON has wide cytotoxicity and could conspicuously do harm to both of the prokaryotic and the eukaryotic cells. At present, the studies are mostly focus on the methods of detection, the immunotoxicology and the effects on the normal cells of DON, while there are few reports of DON on the abnormal ones.To explore the effects of DON on the proliferation of tumor cells and reveal the related mechanism as well as to overall learn the biological action of DON, and provide the experimental evidence for the application, cell cycle and the expression of related proteins such as CyclinE and P21^WAF/CIP1 in two human gastric adenocarcinoma cell lines—SGC-7901(well- differentiated) and BGC-823 (poorly differentiated) were...