| Background: It is necessary to block renal circulation temporarily in some complicated renal operations such as nephropyelolithotomy and nephron sparing nephrectomy of renal tumors. As well as the ischemia-reperfusion injury is the major reason which causes delayed graft function (DGF). How to attenuate renal ischemia-reperfusion injury is a highlight in nephroprotective studies. It was reported that estrogen has protective effect on many organ ischemia-reperfusion injury such as heart, brain, liver. Whether it has protective effect against renal ischemia-reperfusion injury or not, and its mechanism of against induction is not known to us.Objective: To explore the protective effects and the beneficial mechanism of induction after estrogen preconditioning, a model of bilateral post-ischemic renal injury by clamping renal pedicles for 45 minutes was set up, and we examined the renal function, the level of TNF-α in serum by enzyme linked immunosorbent assay (ELISA) method, histology, the ultrastructure of kidney, and the expression of NF-κB by immunohistochemical method.Methods: Male Sprague-Dawley rats(n=75) weighing 300-350g (Experiment Animal Center of Academy of Military Medical Sciences)were used. The rats were divided randomly...
|
PDF Full Text Request