Effect Of Acupuncture On NO,NOS,SOD,MDA And Ultrastructure Of Ovarian Or Ganization Of Menopausal Female Rats

Purpose: This experiment was to investigate effect of acupuncture at "Zu San Li" and "Guan Yuan" on antioxidation ability and ultrastructure of ovarian organization of menopausal female rats.Method: After acupuncture at "Zu San Li" and "Guan Yuan" of menopausal female aged rats, ovarian albumen content was mensurated with Bradford method;ovarian nitrous oxide (NO) content was mensurated with nitrate reductase method;ovarian nitric oxide synthase (NOS) content was mensurated with spectrophotometry method;ovarian Superoxide dismutase (SOD) energy was mensurated with xanthine oxidase method;ovarian tissue malonaldehyde (MDA) was determined with thiobarbituric acid test;and changing of ovarian ultrastructure was detected with electron microscope.Results: with comparing between the acupuncture group and the comparing model group, it can be conclude that acupuncture at at "Zu San Li" and "Guan Yuan" can adjust NO content and NOS activity of ovaries, increase SOD activity, and decrease MDA content, the ovarian ultrastructure of of the menopausal female rats, consequently, was reformed, which was represented by that there were more follicles, mitochondrial, smooth surfaced endoplasmic reticulum, while less atretic follicles and lysosome.Conclusion: acupuncture at "Zu San Li" and "Guan Yuan" of menopausal female aged rats can improve antioxidation ability to eliminate free radical, modulate ovarian physiological function, and refine ultrastructure of ovarian organization, which will delay ovarian consenescence.