Effect Of Acupuncture On Antioxidation And Ultrastructure Of Brain Tissue Of Menopausal Female Rats

Purpose: This experiment was to investigate effect of acupuncture at "Zu San Li" and "Guan Yuan" on antioxidation ability and ultrastructure of cerebullar organization of menopausal female rats.Method: After acupuncture at "Zu San Li" and " Guan Yuan" of menopausal female aged rats, cerebullar albumen content was mensurated with Bradford method; cerebullar nitrogen monoxidum (NO)content was mensurated with nitrate reductase method; cerebullar nitric oxide synthase (NOS) content was mensurated with spectrophotometry method; cerebullar Superoxide dismutase (SOD) energy was mensurated with xanthine oxidase method; cerebullar malonaldehyde (MDA) content was determined with thiobarbituric acid test; and changing of brain tissue ultrastructure was detected with electron microscope.Results: with comparing between the acupuncture group and the comparing model group, with comparing between the acupuncture group and the young model group, it can be conclude that acupuncture at "Zu San Li" and "Guan Yuan" can adjust NO content and NOS activity of brain, increase SOD activity, and decrease MDA content, we find obvious improvement about the ultrastructure of brain tissue of the acupuncture set compared with the comparing model set. It showed that form of nucleus of neuron and nuclear envelop was integral, chromatin was proport, there was more mitochondrion, rough endoplasmic reticulum, Golgi complex, microtubule and microfilament, synapse and synapse vesicle, while less lipofuscin.Conclusion: acupuncture at "Zu San Li" and "Guan Yuan" of menopausal female aged rats can improve antioxidation ability to eliminate free radical, can obviously improve the ultrastructure in brain tissue which will delay brain consenescence.