Effect Of Acupuncture On Antioxidation And Ultrastructure Of Heart Organization Of Menopausal Female Rats

Purpose: This experiment was to investigate effect of acupuncture at "Zu San Li" and "Guan Yuan" on antioxidation ability and ultrastructure of heart organization of menopausal female rats.Method: After acupuncture at "Zu San Li" and "Guan Yuan" of menopausal female aged rats, heart's albumen content was mensurated with Bradford method; heart's nitrous oxide (NO) content was mensurated with nitrate reductase method; heart's nitric oxide synthase (NOS) content was mensurated with spectrophotometry method; heart's Superoxide dismutase (SOD) energy was mensurated with xanthine oxidase method; heart's tissue malonaldehyde (MDA) was determined with thiobarbituric acid test; clearand changing of heart's ultrastructure was detected with electron microscope.Results: with comparing between the acupuncture group and the comparing model group, with comparing between the acupuncture group and the young mordel group,it can be conclude that acupuncture at "Zu San Li" and "Guan Yuan" can adjust NO content and NOS activity of hearts, increase SOD activity, and decrease MDA content, we find obvious improvement about the ultrastructure of heart tissue of the acupuncture set compared the comparison set. It showed that myoneme of cardiac muscle cell was integral, sarcomere was regulation,Z line, M line and H band was clear.In nucleus density of chromatin was proport, distribution of chromatin was uniformity. Karyotheca of cardiac muscle cell and mitochondrial membrane was integrity, mitochondrial cristae was clear.Conclusion: acupuncture at "Zu San Li" and "Guan Yuan" of menopausal female aged rats can improve antioxidation ability to eliminate free radical, modulate heart's physiological function, and refine ultrastructure of heart's organization, which will delay heart's consenescence.