Effects Of Compound 48/80 On The Plaque In The ApoE～(-/-) Mice Aorta And The Study Of Mast Cell On The Migration Of Smooth Muscle Cell

Objective:Mast cells (MCs) are a kind of inflammatory-immune cells. Recently, with the progress of atherosclerosis(AS) etiology, we found that MCs were involved in the every evolutive phase of atherosclerosis. Vascular smooth muscle cells (VSMCs), migrating from the media to the intima, and some inflammatory-immune cells(T lymphocyte, macrophage, mast cells and so on) are the cellar components of as lesions. But the relationship between MCs and VSMCs has not been clarified. Our study used ApoE^-/-mice as the animal model to observe the effects of Compound 48/80 and Ketotifen Fumarate on the lesions of AS, and used the cultured rats VSMCs and the compound 48/80 to clarify the effects of MCs on the movement of VSMCs. And we also discussed the mechanisms of VSMC' s migration primarily.Methods:Thirty ApoE^-/- mice were randomly divided into three groups.There was the control group,the Compound 48/80 group and the Ketotifen Fumarate group. The Compound 48/80 group was intraparentoneal injected 0.15mg/kg Compound 48/80, and the frequency was two times every week; The Ketotifen Fumarate group was given 0.1ml per mouse by Gastric Perfusion, and the frequency was once a day. All the groups were fed by standard laboratory chow for 24 weeks, then all of the animals were euthanized. Blood was sampled from the eye. The serum lipids were Enzymatically detected. Sudan IV was used to stain the thoracic and abdominalis aorta. The vessel was fixed by in situ liquid flow. Thoracic aorta was fixed in 10% formalin, then embedded in paraffin .Serial sections and HE staining were carried out.