Study On The Inhibition By The Abstracts Of Scutellariabarbatae D.Don Of Cancer Angiogenesis

Angiogenesis means the formation of new capillaries from preexisting vessels. In 1970′Science Dr.Judah Folkman hypothesized that angiogenesis was obligatory for tumor growth. A tumor is unable to grow above 2mm3 and then towards into the dormancy period without development of a new blood vessel. When angiogenesis stimulators is stronger than angiogenesis inhibitors, the switch of angiogenesis is on and increases angiogenesis, which result in the proliferation,invasion and metastasis of tumor. Angiogenesis is known to be a complex process that requires endothelial cell proliferation,migration and differentiation into tube-like structures and controlled by extensive interactions between a various peptides and other modulating factors produced by host or tumor cell. Now anti-angiogenesis therapy has become the focus of cancer research. But the research on traditional Chinese medicine targeted to angiogenesis just starts in our country.Systematic solvent separation method and column separation method was employed to extract different parts of Scutellaria barbatae D.DON. The effects of different parts of Scutellaria barbatae D.DON on proliferation of HUVEC were tested by Trypan Blue exclusion and MTT method. Select the most effective part and investigate the mechanism of inhibiting cancer angiogenesis through apoptosis,tube formation experiment,Transwell chamber,ELISA,Griess assay.The main result and conclusion showed as below:1,Experiment of different parts of Scutellaria barbatae D.DON selection: we find the crude extract of Scutellaria barbatae D.DON group and flavonoid A06 group can inhibit proliferation of HUVEC in contrast to the control group, the inhibit effect of...