| Fascin was an actin-binding protein. Some differential proteomics studies in esophageal squamous cell carcinoma (ESCC) had confirmed that fascin was one of the differentially expressed proteins between the tissues of ESCC and normal epithelia. In recent years, the overexpression of fascin in many cancers had been reported and was related with their carcinogenesis, invasion and metastasis. This study further detected the different expression in protein and transcriptional levels, secretion and location of fascin in ESCC, especially in its premalignant lesions, investigated the relationship of the differential expression of fascin and ESCC, and discussed the possibility of fascin as a tumor marker of diagnosis and prognosis.The expression of fascin was tested by Western blot assay in 26 pairs of ESCC and its cancer-adjacent normal tissues. Immunohistochemistry staining was used to test the expression of fascin in esophageal tissues which were endoscopic biopsy from Linzhou city, a high-risk region in Henan province, including 8 cases of normal esophageal epithelia, 54 cases of different pathological classifications of esophageal pre-malignant lesions and 9 cases of advanced ESCC. ELISA analysis was used to measure the level of fascin in the sera of 43 normal and 102 patients of ESCC. RNA extracted from 8 pairs of ESCC and their cancer-adjacent tissues were reversely transcribed into complementary DNA, fascin cDNA was amplified by PCR analysis.Fascin was significantly up-regulated in the ESCC tissues comparing to its cancer-adjacent normal tissues where low or almost no expression of fascin was detected by Western blot analysis. Comparing with normal esophageal epithelia where fascin was expressed in the basal layer, Over-expressed of fascin was detected in most pre-malignant lesions including 85.7% (24/28) from low-grade lesions (mild-dysplasia and moderate-dysplasia) and 96.2% (25/26) from high-grade lesions (severe dysplasia and carcinoma in situ)...
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